STUDY OF ANTIBACTERIAL ACTION MODE OF SILVER NANOPARTICLES SYNTHESIZED USING LIMON (CITRUS LIMON L.) PEEL EXTRACT AND ITS EFFECT ON BACTERIAL CELL MORPHOLOGY
Green synthesized silver nanoparticles (AgNPs) have been widely developed and researched as new antimicrobial agents, but the mechanism of antimicrobial activity of AgNPs is still unknown. In this research, lemon peel extract was used as a source reducing and stabilizing agent in the synthesis of si...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/82465 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Green synthesized silver nanoparticles (AgNPs) have been widely developed and researched as new antimicrobial agents, but the mechanism of antimicrobial activity of AgNPs is still unknown. In this research, lemon peel extract was used as a source reducing and stabilizing agent in the synthesis of silver nanoparticles using microwaves mediated (CL-AgNPs). The synthesized colloid solution CL-AgNPs showed a peak absorbance at a spesific surface plasmon resonance (SPR) wavelength for silver nanoparticles of 415 nm. It was found that CL-AgNPs were stable as indicated by the zeta potential value of -59,6 mv with a diameter size of particle of 52,6 nm. The colloid of green synthesized silver nanoparticle were dried using freeze drying method to obtain solid CL-AgNPs. The green synthesized silver nanoparticles (CL-AgNPs) showed the MIC values against Cutibacterium acnes, Staphylococcus aureus, Pseudomonas aeruginosa, and Streptococcus mutans are 50, 25, 25, and 50 ?g/mL, respectively. Determination of the bacterial time kill curve was carried out by varying the value of 1MIC, 2MIC, 4MIC, and untreated bacterial control, bactericidal activity is indicated when there is a reduction in the number of colonies of more than or equal to 3log10 CFU/mL. The CL-AgNPs value of 2MIC and 4MIC showed a bactericidal activity after 24 hours incubation, although 1MIC showed bacteriostatic activity after 24 hours incubation for Streptococcus mutans. The CL- AgNPs with a value of 4MIC showed a bactericidal activity after 4 hours incubation, the value of 1MIC dan 2MIC showed a bactericidal activities after 8 hours incubation for Pseudomonas aeruginosa. Morphological observations were carried out to determine the effect of 2MIC CL-AgNPs exposure after 18 – 24 hours of incubation at temperature of 37oC on bacterial morphology using SEM instrumentation, morphological damage was observed in the cell walls of gram-positive bacteria (Cutibacterium acnes, Staphylococcus aureus, and Streptococcus mutans) as well as on the cell membrane of gram-negative bacteria (Pseudomonas aeruginosa).
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