FORMULATION OF CELLULOSE ACETATE NANOFIBER CONTAINS BITTER MELON FRUIT EXTRACT (MOMORDICA CHARANTIA L.) WITH EVALUATION OF ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY IN VITRO

• Main Author: Nurrani, Bintang
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82500
• Institution: Institut Teknologi Bandung

Diabetes mellitus (DM) is a disease that has the potential to cause hyperglycemia, resulting in neuropathy and allowing unnoticed wounds to appear. In the wound healing process for DM sufferers, the inflammatory phase is prolonged and uncontrolled. Based on previous research, preparations that can control wounds in diabetes also need to be improved to maintain wound contraction by creating an adhesive layer. To overcome these problems, one alternative is to formulate nanofiber (NF) preparations containing plant extracts, such as paria fruit extract (Momordica charantia L.) (EP), which is expected to increase wound healing activity. The NF-EP formula was developed by optimizing the process with an electrospin tool which was carried out simultaneously with optimizing the concentration of cellulose acetate and EP. Then, nanofiber characterization was carried out, namely organoleptic, particle size, thickness, wetting ability, swelling ability, encapsulation efficiency, and in vitro release; and testing for antioxidant and antibacterial activity was carried out. The optimal process for the electrospin device is with a humidity level of 75%, a distance between the collector and the spinneret of 13 cm, a flow rate of 1 mL/hour, a needle diameter of 0.83 mm, and a voltage of ~14 kV. The optimal formula was 8% cellulose acetate (w/v to solvent), 1% EP (w/w to cellulose acetate), and Ac-DMAc solvent (2:1 v/v). NF-EP is white, has a rougher surface than NF, and feels thinner than NF. Then, the NF-EP characterization is particle size 127.4 ± 29.20 nm, thickness 40.33 ± 2.52 µm, wetting ability 68.13 ± 3.31°, mechanical ability 71.61 ± 2.2416 KPa, swelling ability 4433.86 ± 460.32%, encapsulation efficiency (EE) 70.22 ± 3.47%, and in vitro release close to the Korsmayer-Peppas mathematical model. The antioxidant and antibacterial activity of NF-EP is not better than EP, but NF-EP has an absorption capacity 45 times higher than its initial weight which supports wound healing activity.