DEVELOPMENT OF CURCUMIN DERMAL DELIVERY SYSTEM USING BACTERIAL NANOCELLULOSE MEMBRANE PRODUCED BY ALTERNATIVE SUBSTRATE FROM AGRO-INDUSTRIAL WASTE

• Main Author: Kalpika Resmi, Juniar
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82586
• Institution: Institut Teknologi Bandung

Bacterial nanocellulose (BNC) is an eco-friendly biomaterial useful in the biomedical field as a drug/drug carrier that can be produced using the Hestrin-Schramm medium. The high price of the medium limits its use in industry. This study aims to determine the best substrate component based on the yield value and to evaluate the physical properties and permeation capability of BNC as a drug delivery matrix containing curcumin. The substrate components consisted of 6 groups consisting of siwalan sap (NS) and tofu pulp, which act as a source of glucose and nitrogen in the ratio of 0:100 (S-1), 20:80 (S-2), 40:60 (S-3), 60:40 (S-4), 80-20 (S-5) and 100:0 (S-6) by static culture. Yield calculation and fermentation broth analysis have been performed. Selected substrates were impregnated in CR-DMSO and CR-NE and characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), X-ray fluorescence (XRF) and sorption efficiency (EE) and drug loading. Permeation test of BNC containing curcumin was performed by Franz diffusion and then visually observed by confocal microscope using mouse skin model. In vivo safety testing was performed on mice skin using hematoxylin and eosin (H&E) staining for inflammatory cell infiltration and neovascularization. AAT-NS substrate (0:100, S-6) was the selected substrate component for BNC formation with a yield value of 118.5±0.09 g/L. Based on the physical characterization CR-DMSO and CR-NE can be well impregnated into BNC. Confocal data showed that both formulations were able to penetrate the dermis. There was no evidence of inflammation or neovascularization in BNC/CR-DMSO and BNC/CR-NE.