ISOLATION OF MARKER COMPOUNDS FROM TEMU HITAM RHIZOMES (CURCUMA AERUGINOSA ROXB.)

• Main Author: Luthfi Andanu, Dhymas
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82599
• Institution: Institut Teknologi Bandung

Temu hitam (Curcuma aeruginosa Roxb.) is a plant from the Zingiberaceae tribe which is used as traditional medicine in several Asian countries, including Indonesia. This plant contains essential oils which have antimicrobial activity. Isolation from C. aeruginosa rhizomes produces many monoterpenoid and sesquiterpenoid compounds. Data on chromatographic patterns of compounds from C. aeruginosa in the Indonesian Herbal Pharmacopoeia (FHI) as a standard book for the quality of herbal medicines is not yet available. To complete this data, it is necessary to isolate marker compounds from C. aeruginosa. This research aims to isolate marker compounds from C. aeruginosa rhizomes. This research was carried out through a series of stages, namely extraction, fractionation, subfractionation, purification, purity testing, and isolate characterization. The process begins with extraction of the C. aeruginosa rhizome product in the form of dry extract. Extraction is carried out by the maceration method using a solvent that increases its polarity. The extraction result is an extract that has been separated from its bonds. Followed by fractionation using the vacuum liquid chromatography method to obtain several fractions. The two selected fractions were subfractionated using the radial chromatography method to produce two selected subfractions, namely subfraction A and subfraction B. Subfraction A was purified using the washing method to obtain isolate A. Subfraction B was subfractionated to obtain sub-subfraction B, then purified using the radial chromatography method , isolate B was obtained. The purity of isolates A and B was tested using three mobile phase, two-dimensional, and ultra-performance liquid chromatography thin layer chromatography methods. Isolates A and B were further characterized by specific spotting and ultra performance liquid chromatography using a mass spectrometer detector. Based on the characterization results, it is suspected that isolate A is a compound with the molecular formula C15H22O2 with a molecular structure consisting of four double bonds and one ring. It is suspected that isolate B is a compound with the molecular formula C15H20O2 with a molecular structure consisting of four double bonds and two rings.