ISOLATION AND ANTIMICROBIAL ACTIVITY TEST OF BACTERIA AND FUNGI FROM SEAWEED FROM PAL ANYER BEACH

• Main Author: Crystabel, Grisella
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82611
• Institution: Institut Teknologi Bandung

The increase in antimicrobial resistance cases has led various studies to find new antimicrobial compounds. Many natural resources derived from the sea are now one of the most promising sources of drug search research. This study aims to isolate microbes from seaweed taken from PAL Beach, Anyer and test their antimicrobial activity against Staphylococcus aureus, Eschericia coli, and Candida albicans. The research stages began with the process of isolating microbes from seaweed using YPDA media. The isolates were then purified and fermented. Fermentation of mold isolates began with cultivation using a shaker for 3 days and continued with static fermentation for 3-4 weeks. Fermentation of bacterial isolates was carried out by dynamic fermentation using a shaker for 7 days. The fermentation results were separated between the media and biomass using filtration with a vacuum Buchner for mold isolates and centrifugation for bacterial isolates. The media and biomass obtained were then extracted with ethyl acetate solvent. The dried extracts obtained were screened for activity using the disc diffusion method. Extracts that produced inhibitory diameter were further tested by microdilution and drop test methods to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal/Fungicidal Concentration (MBC/MFC). From the results of the study, 2 mold isolates and 3 bacterial isolates were obtained which were coded FPAL 1, FPAL 2, BPAL 1, BPAL 2, and BPAL 3. The highest activity results were shown by media and biomass extracts of FPAL 1 against S. aureus and C. albicans with MIC values of 250 ?g/mL each. The results of this study indicate that the FPAL 1 fungal isolate has moderate antimicrobial activity and has the potential to become a new antimicrobial source.