SYNTHESIS AND CHARACTERIZATION OF BACTERIAL NANOCELLULOSE CONTAINING PROPOLIS EXTRACT ALONG WITH ANTIMICROBIAL AND IN VIVO BURN WOUND HEALING ACTIVITY TESTS

• Main Author: Tantya Hadiwarsito, Calista
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82616
• Institution: Institut Teknologi Bandung

Bacterial nanocellulose (BNC) is a promising material in the remediation of burn wounds due to the advantages they serve. For instance, these materials are highly biocompatible, biodegradable, and possesses a high purity of cellulose. BNC synthesis requires a rich carbon source, considering its adjustable production method—coconut water was utilized as a carbon source substitute due to its economic value and abundance. Furthermore, the characteristics of BNC fulfills most optimal wound dressing characteristics—requiring minimal modification to achieve optimum wound healing properties. The main modification required for BNC is enhancing its antimicrobial activity. In this study, antimicrobial activity enhancement was achieved by impregnating BNC using nonethanolic propolis extract (PgEP). This study aims to determine the yield of BNC produced from coconut water media; characterize PgEP impregnated BNC (BNC-P) by FTIR, encapsulation efficiency, and drug load; determine the antimicrobial activity of BNC-P against Staphylococcus aureus; and demonstrate the wound healing activity of BNC-P on in vivo burn wound. Using coconut water as a carbon source substitute yields 161,54 ± 35,92 g/L which was significantly greater than standard media (p = 0,025). BNC-P was observed to have an encapsulation efficiency of 52,91 ± 1,16% and drug load is directly proportional to PgEP concentration. Regarding its antimicrobial activity, BNC-P at three different concentrations (1, 3, and 5%) exhibited an inhibition diameter of 7,7 ± 0,31, 12,22 ± 0,99, and 13,68 ± 1,58 mm respectively. Whereupon the wound healing demonstration on in vivo burn wound, BNC-P with concentration of 1% (BNC-P1) was determined to have the greatest burn wound healing activity.