FRACTIONATION AND ANTIBACTERIAL ACTIVITY TEST OF SECONDARY METABOLITES FROM MARINE BACTERIA BACILLUS VELEZENSIS
Increasing bacterial resistance is a serious concern for many countries, including Indonesia. The use of early antibiotics is becoming increasingly ineffective in suppressing bacteria, prompting many health experts to search for new candidate antibiotic compounds. The search for new candidate com...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/82617 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Increasing bacterial resistance is a serious concern for many countries, including Indonesia. The use
of early antibiotics is becoming increasingly ineffective in suppressing bacteria, prompting many
health experts to search for new candidate antibiotic compounds. The search for new candidate
compounds is widely conducted in various ecosystems, one of which is the sea because the diversity
of living organisms and extreme environmental conditions allow the isolation of new compounds
that are active antimicrobials. Bacillus velezensis is one of the bacteria from the collection of the
Microbiology Laboratory of the School of Pharmacy ITB and is known to have antimicrobial activity
against Gram-negative bacteria. This study was conducted to determine the subfraction responsible
for the antibacterial activity against Escherichia coli, Salmonella typhi, and Pseudomonas
aeruginosa. The research included bacterial rejuvenation, bacterial identification, bacterial
fermentation, extraction of secondary metabolites, fractionation of secondary metabolites,
subfractionation of secondary metabolites, and testing of antibacterial activity by disk diffusion and
microdilution methods to determine the value of minimum inhibitory concentration (MIC),
followed by drop test to determine the value of minimum bactericidal concentration (MBC). The
results showed that biomass extract, subfraction 6, and subfraction 7 each showed antibacterial
activity at a concentration of 1024 ?g/mL against Escherichia coli. Other results showed that
subfraction 5 showed antibacterial activity at a concentration of 1024 ?g/mL against Salmonella
typhi, but no extract, fraction, or subfraction showed antibacterial activity at all concentration
series tested against Pseudomonas aeruginosa. All extracts, fractions, and subfractions that
provided antibacterial activity were weak and bacteriostatic.
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