ISOLATION AND ANTIMICROBIAL ACTIVITY OF ENDOPHYTIC BACTERIA AND FUNGI IN SEAGRAPE (CAULERPA SP.) FROM PAL BEACH ANYER

• Main Author: Samantha Untoro, Maria
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82618
• Institution: Institut Teknologi Bandung

Antimicrobial resistance is a global health problem with high cases of mortality. Conducting research on alternative antimicrobial sources is one of the solutions to this problem. Seagrapes (Caulerpa sp.) are green algae rich in bioactive compounds which have potential as a source of alternative antimicrobial compounds. The aim of this study is to isolate endophytic microbes in seagrape obtained from PAL Beach, Anyer and test their antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Candida albicans. Endophytic microbes were isolated using direct planting technique and observed for their macroscopic and microscopic features. Bacterial isolates were fermented for 7 days whereas fungal isolates were fermented for 21 days. Biomass and media were separated using centrifugation and filtration. Biomass was extracted by maceration and media was extracted by liquid-liquid extraction using ethyl acetate. Dry extracts were tested for their antimicrobial activity by agar diffusion method followed by microdilution method to determine their Minimum Inhibition Concentration (MIC). Minimum Killing Concentration (MKC) was then determined by drop test. As a result, five isolates were obtained consisting of 2 bacterial isolates (B-AGL-1, B-AGL-2), 1 yeast isolate (R-AGL-1), and 2 mold isolates (K-AGL-1, K-AGL-2). The highest antimicrobial activities were shown by the biomass extract of B-AGL-1 against E. coli dan S. aureus with MIC value of 64 µg/mL classified as strong activity and the media extract of B-AGL-1 against S. aureus with MIC value of 128 µg/mL classified as moderate activity. Thus, endophytic bacterial extracts from seagrapes have potential as new antimicrobial sources.