ANTIBACTERIAL ACTIVITY OF EXTRACT AND FRACTION FROM ENDOPHYTIC FUNGI EXOPHIALA PISCIPHILA S14

• Main Author: Josephine
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82643
• Institution: Institut Teknologi Bandung

Antibiotic resistance is a problem that will continuously occur in the world. In 2019, deaths caused by antibiotic resistance has reached about 4.95 million people. One of the attempts to address this problem is developing and researching new antibacterial substances. Exophiala pisciphila is a dark septate endophyte that can grow in a heavy stress condition, and its secondary metabolites are suspected to have antibacterial properties, so its potential as an antibacterial can be determined. First, E. pisciphila liquid culture was separated to its biomass and filtrate using sentrifugation. Biomass obtained was then extracted using maceration method with methanol and the filtrate was extracted by liquid-liquid extraction with ethyl acetate. Through antibacterial activity screening with the disk diffusion method, the ethyl acetate extract of Exophiala pisciphila has shown an inhibition zone of 9,20 ± 0,4 mm towards Escherichia coli and 9,65 ± 0,25 mm towards Staphylococcus aureus. Between all of the obtained fractions, the semipolar fraction of E. pisciphila has the best antibacterial activity determined with the microdilution method. The fraction has a minimum inhibitory concentration of 1024 µg/mL towards E. coli and S. aureus and a minimum bactericidal concentration of 2048 µg/mL towards S. aureus. Compounds identified in the fraction are phenolics and steroids or triterpenoids with the best activity against gram-positive bacteria.