DEVELOPMENT OF A MOUTHWASH FROM HYDROSOL OF THYME HERB (THYMUS VULGARIS) AND ANTIBACTERIAL ACTIVITY TEST AGAINST STREPTOCOCCUS MUTANS

• Main Author: Diah Laksmi Puspitasari, Made
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82688
• Institution: Institut Teknologi Bandung

Dental caries is a common oral disease caused by Streptococcus mutans. To prevent dental caries, a preventive measure is needed, one of which is rinsing the mouth with mouthwash. However, many mouthwashes contain chlorhexidine, which gives side effects in the form of tooth discoloration and a decrease in salivary pH, which will be a problem for the balance of oral microbiota. Thyme herb (Thymus vulgaris) is one of the plants with good antibacterial activity, especially essential oils. In the production of essential oil by steam distillation, there is distillate water or hydrosol, considered production waste. From 100 grams of simplisia and 1500 mL of distilled water was distillated and the first 300 mL of hydrosol had antibacterial activity based on the results of the disc diffusion test. The microdilution test showed that the hydrosol has MIC and MBC at 50% concentration. The phenol coefficient test was carried out to determine the concentration of hydrosol in the formulation, obtained a concentration of 45% can still kill bacteria so the concentration was chosen and continued to the formulation. The mouthwash formulation was hydrosol, saccharin sodium, propylparaben, methylparaben, sodium citrate, citric acid, ethanol, glycerin, mint flavor, and distilled water. A pH of hydrosol mouthwash was 5.87±0.02 with a viscosity 0.99 mPa.s was obtained for the final preparation. The hedonic test conducted gave the result that mouthwash still needs to be improved regarding its smell and freshness. The confirmation test showed that the antiseptic activity of a commercial mouthwash was better than hydrosol mouthwash, but the contact test showed that hydrosol mouthwash has a bactericidal ability because it has a killing power of 99.9% at a contact time of 120 seconds.