ENRICHMENT METHOD OF XANTHORRHIZOL AND CURCUMINOID IN TEMULAWAK (CURCUMA XANTHORRHIZA ROXB.) AS ANTIBACTERIAL AGENT

• Main Author: Audrey Kurniawan, Claudia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82691
• Institution: Institut Teknologi Bandung

Temulawak (Curcuma xanthorrhiza Roxb.) is used massively in Indonesia for medicinal, food and natural dye needs. The best known pharmacological ac@vi@es of temulawak are as an@microbial, an@bacterial, an@oxidant and an@-inflammatory. The main compounds of secondary metabolites of temulawak which act as an@bacterials are xanthorrhizol and curcumin. This study aims to determine the method of enrichment of curcuminoid (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and xanthorrhizol and to test the an@bacterial ac@vity of temulawak isolates against the growth inhibi@on of Propionibacterium acnes and Pseudomonas aeruginosa. A comparison of macera@on and percola@on extrac@on methods was used. Extract was con@nued with frac@ona@on and isola@on using vacuum liquid chromatography (VLC) and radial chromatography (RC) methods. The macera@on method is e?ec@ve in enriching demethoxycurcumin, bisdemethoxycurcumin, xanthorrhizol, while the percola@on method is e?ec@ve in enriching curcumin compounds. Determina@on of the minimum inhibitory concentra@on (MIC) value for bacteria was carried out using the microdilu@on method with a sample concentra@on of 4–1024 µg/mL with posi@ve control tetracycline HCl. The most e?ec@ve an@microbial compound against P. acnes bacteria is xanthorrhizol (MIC 8; MBC 16 µg/mL) and the most e?ec@ve against P. aeruginosa bacteria is curcumin (MIC 16; MBC 128 µg/mL).