OPTIMATION OF TEMPERATURE AND C:N MOLAR RATIO IN HIGH FRUCTOSE CORN SYRUPE FERMENTATION USING CLOSTRIDIUM ACETOBUTYLICUM FOR BIO-BUTANOL PRODUCTION

• Main Author: Victoria Fernandez, Sheencia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/82780
• Institution: Institut Teknologi Bandung

Butanol is a four carbon alcohol used as solvent in pharmaceuticals and cosmetic industries, raw material for butyl acrylate and methacrylate, and alternative fuel. Butanol production is usually done in two ways, from petroleum refining and acetone-butanol-ethanol (ABE) fementation by Clostridium sp. Butanol production from petroleum is heavily affected by fossil oil price and causes environmental problems such as global warming, therefore bio-butanol production from ABE fermentation by Clostridium sp. poses as more sustainable alternative. High fructose corn syrup is chosen as the fermentation substrate because of its relatively low price and high content of simple sugars which leads to high biomass yield. This research carried out fermentation temperature and media C:N molar ratio optimization to maximize bio-butanol production. Based on the results, the optimum temperature for bio-butanol production is 37? with media C:N molar ratio of 45, whereas the optimum temperature and media C:N molar ratio for maximum biomass yield is 37? and C:N molar ratio of 65. Bio-butanol production done using C:N molar ratio of 45 is 3,42 g/L. Production done in 8 L vessel with temperature of 37? and C:N molar ratio of 65 resulted in 0,6 g/L of bio-butanol and cell dry weight of 1,21 g/L.