OPTIMIZATION OF TEMPERATURE AND MEDIUM FRUCTOSE CONCENTRATION FOR BIOETHANOL PRODUCTION FROM FERMENTATION OF HIGH FRUCTOSE CORN SYRUP WITH SACCHAROMYCES CEREVISIAE

Energy needs continue to increase significantly from time to time along with the growth of human population. Energy needs are expected to increase by 15% throughout 2023 to 2050. The rising need for energy will result in increased use of fossil fuels which contribute greatly to greenhouse gas (GH...

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Bibliographic Details
Main Author: Fawzya Yazmin, Shelby
Format: Final Project
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/82782
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Institution: Institut Teknologi Bandung
Language: Indonesia
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Summary:Energy needs continue to increase significantly from time to time along with the growth of human population. Energy needs are expected to increase by 15% throughout 2023 to 2050. The rising need for energy will result in increased use of fossil fuels which contribute greatly to greenhouse gas (GHG) emissions and global warming. Besides their environmental harm, fossil fuels are estimated to run out in 40-50 years. A more environmentally friendly alternative energy that could be considered for this energy demand is bio-ethanol. Bio-ethanol is generally produced by fermenting biomass containing sugar by microorganisms. The microorganism that is often used for alcoholic fermentation is Saccharomyces cerevisiae because of its high tolerance to ethanol, can grow in the pH range of 4- 6, easily ferments various types of sugar and is often used in industry. Sugar used as a fermentation substrate can come from sugar refinery waste or milling process waste, such as high fructose corn syrup (HFCS). In this research, fermentation optimization of corn processing by-products in the form of HFCS was carried out to produce bio-ethanol. The optimization process was carried out to determine the most optimum temperature and fructose concentration of HFCS. Then, up-scaling was carried out on an 8L scale with optimal environmental temperature conditions and fructose concentration. Based on research, the optimum fermentation temperature is 31?C and the best concentration of fructose from HFCS in the fermentation medium used is 8% fructose. The up-scale results on the 8L scale show that the total bio-ethanol fermentation results reached up to 266,96 g/L.