OPTIMIZATION OF TEMPERATURE AND MEDIUM FRUCTOSE CONCENTRATION FOR BIOETHANOL PRODUCTION FROM FERMENTATION OF HIGH FRUCTOSE CORN SYRUP WITH SACCHAROMYCES CEREVISIAE
Energy needs continue to increase significantly from time to time along with the growth of human population. Energy needs are expected to increase by 15% throughout 2023 to 2050. The rising need for energy will result in increased use of fossil fuels which contribute greatly to greenhouse gas (GH...
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Format: | Final Project |
Language: | Indonesia |
Online Access: | https://digilib.itb.ac.id/gdl/view/82782 |
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Institution: | Institut Teknologi Bandung |
Language: | Indonesia |
Summary: | Energy needs continue to increase significantly from time to time along with the
growth of human population. Energy needs are expected to increase by 15%
throughout 2023 to 2050. The rising need for energy will result in increased use of
fossil fuels which contribute greatly to greenhouse gas (GHG) emissions and global
warming. Besides their environmental harm, fossil fuels are estimated to run out in
40-50 years. A more environmentally friendly alternative energy that could be
considered for this energy demand is bio-ethanol. Bio-ethanol is generally
produced by fermenting biomass containing sugar by microorganisms. The
microorganism that is often used for alcoholic fermentation is Saccharomyces
cerevisiae because of its high tolerance to ethanol, can grow in the pH range of 4-
6, easily ferments various types of sugar and is often used in industry. Sugar used
as a fermentation substrate can come from sugar refinery waste or milling process
waste, such as high fructose corn syrup (HFCS). In this research, fermentation
optimization of corn processing by-products in the form of HFCS was carried out
to produce bio-ethanol. The optimization process was carried out to determine the
most optimum temperature and fructose concentration of HFCS. Then, up-scaling
was carried out on an 8L scale with optimal environmental temperature conditions
and fructose concentration. Based on research, the optimum fermentation
temperature is 31?C and the best concentration of fructose from HFCS in the
fermentation medium used is 8% fructose. The up-scale results on the 8L scale show
that the total bio-ethanol fermentation results reached up to 266,96 g/L. |
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