THE EFFECT OF HYDROTHERMAL PRETREATMENT AND SOLID-STATE FERMENTATION USING TRICHODERMA VIRIDE ON LIGNOCELLULOSE DEGRADATION AND HUMIC ACID SYNTHESIS FROM CORN COB WASTE (ZEA MAYS L.)
Corn is one of the staple food commodities in Indonesia, with production reaching 22.5 million tons per year. However, corn cobs are often discarded as waste with an estimated annual amount of around 5.7 million tons and this is expected to increase. Corn cobs contain a significant amount of lign...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/82902 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Corn is one of the staple food commodities in Indonesia, with production reaching
22.5 million tons per year. However, corn cobs are often discarded as waste with
an estimated annual amount of around 5.7 million tons and this is expected to
increase. Corn cobs contain a significant amount of lignocellulosic components,
consisting of approximately 20-30% cellulose, 35-40% hemicellulose, and 15-20%
lignin. The lignocellulosic components can be degraded through fermentation using
the biological agent like Trichoderma viride and used in the formation of humic
acid. An initial treatment of hydrothermal is carried out to break down part of the
lignocellulose structure and enlarge the biomass's pores. This study aimed to
determine the effect of solid substrate fermentation time by Trichoderma viride on
the lignocellulosic content of corn cobs and the yield of humic acid, with variations
in initial hydrothermal treatment times of 30, 45, and 60 minutes. Fermentation was
conducted for 35 days in a closed and dark room with variations in time intervals
of 7, 14, 21, 28, and 35 days. The lignocellulosic content decreased during the 35-
day fermentation period with variations in initial hydrothermal treatment times,
with the lowest content found at the 60-minute initial hydrothermal treatment time.
The humic acid content of corn cobs increased during the 35-day fermentation
period with variations in initial hydrothermal treatment times. The highest humic
acid content was obtained on the 35th day of fermentation with a 60-minute initial
hydrothermal treatment time, reaching 6.5%. Correlation analysis results indicated
that cellulose degradation significantly influenced humic acid formation, while
hemicellulose and lignin degradation had the opposite effect. |
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