GREEN SYNTHESIS AND CHARACTERIZATION OF AG/MGO NANOCOMPOSITE AS ACTIVE COMPOUND FOR FREE-ANTIBIOTICS WOUND DRESSING
The use of antibiotics in wound management can cause allergic reactions in some patients. The need for rapid wound treatment has driven the search for alternative methods that avoid allergic reactions while still preventing bacterial infections. Silver nanoparticles (Ag) are known for their ef...
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| Format: | Final Project |
| Language: | Indonesia |
| Subjects: | |
| Online Access: | https://digilib.itb.ac.id/gdl/view/82932 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The use of antibiotics in wound management can cause allergic reactions in some patients.
The need for rapid wound treatment has driven the search for alternative methods that avoid
allergic reactions while still preventing bacterial infections. Silver nanoparticles (Ag) are
known for their effective antibacterial properties that reduce bacterial activity and have been
widely applied in the medical field. However, continuous exposure of body cells to silver
nanoparticles has been identified to cause toxic reactions, particularly in the dermis layer of
the skin. In addition to silver, magnesium oxide (MgO) also has antibacterial properties,
though with lesser effectiveness, and is not sufficient to prevent infections in wounds on its
own. Ag/MgO nanocomposites are expected to reduce the toxicity of silver nanoparticles and
enhance the antibacterial ability of MgO. This results in antibacterial properties that can be
safely used on wounds without the risk of causing cell damage around the wound.
Furthermore, MgO has been reported to accelerate wound closure. This study aims to
synthesize Ag/MgO nanocomposites through green synthesis using palm leaf extract (Elaeis
guineensis), which is rich in organic compounds such as flavonoids and phenolics that can
play a role in reducing metal nanoparticles. The surface plasmon resonance characteristic of
Ag nanoparticles at a maximum absorption wavelength of around 440 nm was identified and
analyzed using UV-Vis spectrophotometry, while the crystal phase characterization of the
nanocomposite was performed using powder X-ray diffraction (PXRD), revealing overlapping
phases of Ag and MgO at 2? = 38.12°; 44.28°; and 64.42°. The morphology and atomic ratio
of the nanocomposite were scanned using scanning electron microscopy equipped with energy
dispersive spectroscopy (SEM-EDS), showing that the nanocomposite had a flake-like shape
with thickness in the nano range and an atomic ratio of 3:5 Ag/Mg. The antibacterial properties
of the nanocomposite were tested in vitro using the disk diffusion method against
Staphylococcus aureus (gram-positive) and Escherichia coli (gram-negative) bacteria,
demonstrating that the antibacterial properties of the Ag/MgO nanocomposite increased with
the addition of silver precursor concentration during synthesis. The Ag/MgO nanocomposite
exhibited stronger antibacterial properties than MgO and weaker than silver nanoparticles,
indicating that the Ag/MgO nanocomposite is safer to use than silver nanoparticles as an active
agent in preventing bacterial growth in wounds. |
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