SYNTHESIS OF SILICA MODIFIED BY N-[3- (TRIMETHOXYSILYL)PROPYL]ETHYLENEDIAMINE AS HIGH PERFORMANCE LIQUID CHROMATOGRAPHY COLUMN FILLER MATERIAL

• Main Author: Zacharias Sanbra, Dante
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/82989
• Institution: Institut Teknologi Bandung

Propylamine silica particles are often used in chromatography columns for sugar analysis. In this study, a silica column filler material with two amine groups was synthesized. The precursors used were tetraethylorotosilicate (lEOS) and N-[3- (Trimethoxysilyl)Propyl] Ethylenediamine (N-TMSPen). The synthesis method used is the St()ber process, with characterization of the synthesis results using Fourier Transform Infrared (FTIR) and Scanning Electron Microscope (SEM). Methoxytrimethylsilane {TMS) compound was also used in the modification to react the remaining -OH groups to improve the column performance. FTIR characterization showed that silica has been successfully synthesized with the presence of -OH peak (arround 3000 cm-1 , Si-0 peak on silanol (937 cm-1 and Si-0 on ) ) siloxane (1105 cm-1and 813 cm-1 and C-H peak (2980-3050 cm-1 .However, the primary N­ ) ) H peak (3360-3450 cm-1 , secondary N-H peak (3350-3310 cm-1 , and N-H bending peak ) ) (arround 1620 cm-1 are not clearly visible. While SEM results show that the resulting silica particle sizeranges from 1.7-2 µm. The modified silica was then packed onto a HPLC column with a size of 4.6 x 50 mm and the column performance was tested using a HPLC equipped with an RI detector (Reflective Index Detector) for sugar samples. Column performance testing was carried out with a variation of Acetonitrile:Water eluent ratio at a flow rate of 0.5 and 1mL/min showed that the column was not able to separate the sugar mixture