BIODIESEL PRODUCTION FROM BLACK SOLDIER FLY LARVAE (HERMETIA ILLUCENS) THROUGH IN SITU INTERESTERIFICATION ON A CONTINUOUS CENTRIFUGAL REACTOR SYSTEM WITH BIOCATALYST FROM RHIZOPUS ORYZAE

• Main Author: Naila Salsabila, Najwa
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/83003
• Institution: Institut Teknologi Bandung

As an alternative to energy and environmental problems, biodiesel production can be carried out using black soldier fly larvae (BSFL) biomass as a source of animal oil to avoid competition with the food sector. However, in the production of biodiesel, the use of chemical or acid catalysts can cause problems due to the formation of soap in the transesterification process, damage to equipment because it is corrosive, produces a lot of waste water, and causes the complex process of separating the catalyst dissolved in biodiesel. Therefore, the use of lipase enzyme from Rhizopus oryzae biocatalyst with loofah matrix can avoid the negative impact that will be caused to the environment because it can be used many times and does not cause difficult separation with the biodiesel obtained. This study aims to determine the synthesis and enzymatic activity value of Rhizopus oryzae biocatalyst used in the process of in situ interesterification in BSFL with methyl acetate solvent at variation of reaction time of 4, 8, 12, 16, 20, 24, and 28 hours as well as determining the composition and quality of biodiesel obtained from optimum conditions in the continuous centrifugal contactor separator reactor system (CCCS). The synthesized lipase enzyme from R. oryzae biocatalyst showed an enzymatic activity of 4014.945 U/g which was higher than the commercial lipase enzyme. The biodiesel yield value as a function of reaction time from the in situ interesterification process using methyl acetate solvent produced an increasing yield profile until the reaction time of 16 hours and tended to stagnate at reaction times of 20 to 28 hours with the highest biodiesel yield of 94.49% obtained when the reaction time was 28 hours, the rotor speed was 2100 rpm, and the reaction temperature was 37?. The composition of biodiesel is free glycerol, Monoglyceride (MAG), Diglyceride (DAG), Triglyceride (TAG), and Fatty Acid Methyl Ester (FAME) obtained was 0,031%; 0.272%; 4.704%; 0.670%; and 94.323%, respectively. Then, the quality of biodiesel in the optimum reaction conditions, in the form of moisture content, density, kinematic viscosity, and HHV value had values of 0.038%; 884.579 kg/m3; 3.531 mm2/s; and 40.385 MJ/kg, respectively. In this study, it was found that the synthesis of lipase enzyme from Rhizopus oryzae biocatalyst using loofah matrix showed good results through higher enzymatic activity values than commercial lipase and biodiesel quality that met the standards of SNI 7182: 2015 and EN 14214