OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)

Rhodotorula toruloides is an oleaginous yeast capable of accumulating lipid compounds up to 60% by utilizing various agricultural wastes as its nutrient source. The optimization of lipid production systems from Rhodotorula toruloides fermentation can be carried out using response surface methodol...

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Main Author:	Abigail, Adrienne
Format:	Final Project
Language:	Indonesia
Online Access:	https://digilib.itb.ac.id/gdl/view/83005
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Institution:	Institut Teknologi Bandung
Language:	Indonesia

id	id-itb.:83005
spelling	id-itb.:830052024-07-29T15:45:49ZOPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM) Abigail, Adrienne Indonesia Final Project Rhodotorula toruloides, lipid, C/N ratio, inoculum concentration, response surface methodology INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/83005 Rhodotorula toruloides is an oleaginous yeast capable of accumulating lipid compounds up to 60% by utilizing various agricultural wastes as its nutrient source. The optimization of lipid production systems from Rhodotorula toruloides fermentation can be carried out using response surface methodology (RSM) to determine the optimal conditions for the interaction of independent variables. In this study, the interaction of the C/N ratio and inoculum concentration variables will be optimized to obtain biomass production rate, maximum biomass dry weight, and intracellular lipid yield. This research aims to determine the optimal conditions and predicted response values using RSM at a pH of 6,5. Based on the research conducted, the biomass production rate is influenced by the C/N ratio linearly and quadratically. The optimum biomass production rate is achieved with C/N ratio of 3,74 and inoculum concentration of 108 cells/mL. The maximum biomass dry weight is influenced linearly by both the C/N ratio and the inoculum concentration. The optimum biomass dry weight is obtained with C/N ratio of 3,74 and inoculum concentration of 108 cells/mL. The C/N ratio and inoculum concentration variables do not significantly affect the intracellular lipid yield. The optimization process was conducted through the responses of biomass production rate and maximum biomass dry weight. Through RSM analysis, the optimization results for the biomass production rate and biomass dry weight responses show similar responses. The optimum conditions of the optimization results are achieved with C/N ratio of 3,74 and inoculum concentration of 108 cells/mL. The predicted values for the optimized biomass production rate, biomass dry weight, and intracellular lipid yield are 0,09 g/L·h; 13,37 g/L; and 67,23% (w/w), respectively text
institution	Institut Teknologi Bandung
building	Institut Teknologi Bandung Library
continent	Asia
country	Indonesia Indonesia
content_provider	Institut Teknologi Bandung
collection	Digital ITB
language	Indonesia
description	Rhodotorula toruloides is an oleaginous yeast capable of accumulating lipid compounds up to 60% by utilizing various agricultural wastes as its nutrient source. The optimization of lipid production systems from Rhodotorula toruloides fermentation can be carried out using response surface methodology (RSM) to determine the optimal conditions for the interaction of independent variables. In this study, the interaction of the C/N ratio and inoculum concentration variables will be optimized to obtain biomass production rate, maximum biomass dry weight, and intracellular lipid yield. This research aims to determine the optimal conditions and predicted response values using RSM at a pH of 6,5. Based on the research conducted, the biomass production rate is influenced by the C/N ratio linearly and quadratically. The optimum biomass production rate is achieved with C/N ratio of 3,74 and inoculum concentration of 108 cells/mL. The maximum biomass dry weight is influenced linearly by both the C/N ratio and the inoculum concentration. The optimum biomass dry weight is obtained with C/N ratio of 3,74 and inoculum concentration of 108 cells/mL. The C/N ratio and inoculum concentration variables do not significantly affect the intracellular lipid yield. The optimization process was conducted through the responses of biomass production rate and maximum biomass dry weight. Through RSM analysis, the optimization results for the biomass production rate and biomass dry weight responses show similar responses. The optimum conditions of the optimization results are achieved with C/N ratio of 3,74 and inoculum concentration of 108 cells/mL. The predicted values for the optimized biomass production rate, biomass dry weight, and intracellular lipid yield are 0,09 g/L·h; 13,37 g/L; and 67,23% (w/w), respectively
format	Final Project
author	Abigail, Adrienne
spellingShingle	Abigail, Adrienne OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)
author_facet	Abigail, Adrienne
author_sort	Abigail, Adrienne
title	OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)
title_short	OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)
title_full	OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)
title_fullStr	OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)
title_full_unstemmed	OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)
title_sort	optimization of c/n ratio and inoculum concentration in lipid production from rhodotorula toruloides fermentation using two-stage cultivation and response surface methodology (rsm)
url	https://digilib.itb.ac.id/gdl/view/83005
_version_	1822997911247519744

OPTIMIZATION OF C/N RATIO AND INOCULUM CONCENTRATION IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES FERMENTATION USING TWO-STAGE CULTIVATION AND RESPONSE SURFACE METHODOLOGY (RSM)

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