OPTIMIZATION OF FED-BATCH SYSTEM IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES USING HIGH CONCENTRATION PALM SUGAR AS CARBON SOURCE

• Main Author: Pandu Satrio, Dwiko
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/83006
• Institution: Institut Teknologi Bandung

The area of oil palm plantations in Indonesia in 2023 reached 16.8 million hectares (ha). It is undeniable that the palm oil industry has an important role in improving the country's economy because it can attract consumers to foreign countries. However, palm oil plantations can produce solid waste such as palm trunks. The trunks of oil palm trees contain nira with 11.6% (v/v) sucrose, 2.32% (v/v) glucose, and 1.47% (v/v) fructose. This palm sap can be used as palm sugar, which can be utilized as an alternative carbon source for lipid production from Rhodotorula toruloides. In this study, lipid production from Rhodotorula toruloides was carried out using the fed-batch method. The advantages of the fed-batch method are that it requires a shorter time, increases cell concentration and biomass accumulation, and minimizes the occurrence of substrate inhibition or inhibition of the formation of the final product. The strategy used in this study is to feed a very high concentrated limiting substrate (fixed volume). Limiting substrates that affect lipid production are carbon and nitrogen. Therefore, this study aims to determine the optimum feed component for lipid production from Rhodotorula toruloides cultivated in fed-batch using high concentration palm sugar as a carbon source. This study was conducted by varying the feed components, namely feed N, feed C, and feed CN. Feeding was done twice, at the 48th and 96th hours. The optimum rate of biomass increase was found in the CN feed treatment at a rate of 0.134 g/L. Hours with the highest dry biomass of 19.38 g/L. The highest to lowest intracellular lipid yield was 59.13% (w/w) for feed CN, 49.16% (w/w) for feed N, and 22.59% (w/w) for feed C, respectively. The optimum efficiency of the fed-batch cultivation process was found in the CN feed treatment with a total lipid gain (intracellular and extracellular) of 5.12 g. The optimum efficiency of the fed-batch cultivation process was found in the CN feed treatment.