CARBON PASTE ELECTRODE MODIFIED WITH TIO2 AND MOLECULARLY IMPRINTED POLYPYROLE FOR TBHQ ANALYSIS BY VOLTAMMETRY
Tert-butylhydroquinone (TBHQ) is an antioxidant compound that is often used in the food and beverage industry to extend shelf life and prevent oxidation. The reasonable limit for the use of TBHQ antioxidants set by the Indonesian National Standard (SNI 01-0222-1995) is no more than 200 mg/kg....
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/83128 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Tert-butylhydroquinone (TBHQ) is an antioxidant compound that is often used in the food and
beverage industry to extend shelf life and prevent oxidation. The reasonable limit for the use
of TBHQ antioxidants set by the Indonesian National Standard (SNI 01-0222-1995) is no more
than 200 mg/kg. This research aims to develop a carbon paste electrode (EPK) modified with
TiO2 and molecularly imprinted polypyrrole to detect TBHQ voltammetrically. TiO2 was used
to increase electrode sensitivity while Molecularly Imprinted Polymer (MIP) polypyrrole was
used as a selective element to recognize and improve electrode performance for TBHQ
detection. Based on research, EPK modified with TiO2 via the drop method on the EPK surface
with a concentration of 15 mg/mL TiO2 and a TBHQ:Ppy composition of 1:3 with a total of
15 electropolymerization cycles is the optimum composition capable of increasing the
sensitivity and selectivity of EPK against TBHQ based on current. the highest oxidation peak
produced. The optimum condition for measuring TBHQ is TBHQ in PBS pH 7 using the
square wave voltammetry technique. Determination of electrode performance is seen from the
repeatability of TBHQ measurements using 1 EPK (relative standard deviation 2.127) and the
repeatability of making 3 EPKs (relative standard deviation 3.111). Scan rate analysis shows
that the processes occurring on the electrode surface are controlled by diffusion processes.
This modified EPK has three working concentration ranges, namely, 0.001-0.01mM
(R2=0.995), 0.01-0.1 mM (R2=0.991), and 0.1-1 mM (R2=0.991), with limits detection of
0.455 ?M. Meanwhile, TBHQ measurements in instant noodle seasoning oil samples gave
results of 86.21 mg/kg and 91.07 mg/kg respectively for the voltammetry and HPLC methods.
Thus, TiO2 modified EPK and molecularly imprinted polypyrrole can be used to analyze
TBHQ voltammetrically with good performance. |
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