OPTIMIZATION OF FED-BATCH SYSTEM IN LIPID PRODUCTION FROM RHODOTORULA TORULOIDES USING LOW CONCENTRATION PALM SUGAR AS CARBON SOURCE

• Main Author: Retno Khalisha, Nayla
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/83130
• Institution: Institut Teknologi Bandung

The increase in the palm oil industry is accompanied by an increase in waste from oil palm plants. This waste can be utilized as palm brown sugar. Palm brown sugar can be used as an economical carbon source for various industries such as lipid production using R. toruloides yeast. The production system is carried out using a fed-batch system because it is considered to maximize lipid production by minimizing the use of substrates compared to the batch system. The fed-batch strategy used in this study is fixed volume by providing feed in the form of limiting nutrients with high concentrations. One of the important factors in lipid accumulation in biomass is the feeding component used. This study aims to evaluate the optimum feeding component in lipid production using R. toruloides. The three treatment variations were made with initial conditions in the form of a mixture of medium and palm sugar solution as a carbon source with a concentration of 13.72 g/L. Ammonium feeding at initial conditions and feeding was carried out for N and CN variations. Giving palm brown sugar during feeding was done for C and CN variations. This study was conducted with palm sugar preparation, yeast culture preparation, medium preparation, fed-batch cultivation, cell harvesting, cell drying, lipid extraction from dry biomass, and extracellular lipid separation from supernatant. Then CN variation resulted in optimum lipid production using Rhodotorula toruloides yeast with fed-batch cultivation. The maximum CDW produced by harvesting on day 7 was 18.18 g/L with the rate of biomass increase, intracellular lipid gain, and total lipid mass having the highest values with sequential values of 0.09 ± 0.008 g/L.h, 55.58% (w/w), 4.74 g from a culture with a working volume of 300 mL. Then, system engineering using the fed-batch method with this optimum variation can increase lipid yield by 56.22% from lipid yield with the batch method.