PRODUKSI MINYAK NILAM (PATCHOULI OIL) MELALUI KULTUR PUCUK POGOSTEMON CABLIN (BENTH.) DALAM BIOREAKTOR TIS RITA® DENGAN PENAMBAHAN ELISITOR METIL JASMONAT
The patchouli plant (Pogostemon cablin Benth.) is a patchouli oil producing plant. Patchouli oil is composed of several secondary metabolites including terpenoids, flavonoids, alkaloids, steroids and phenylpropanoid glycosides. Thus, the oil has strong pharmacological activity and can be used as...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/83165 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The patchouli plant (Pogostemon cablin Benth.) is a patchouli oil producing plant.
Patchouli oil is composed of several secondary metabolites including terpenoids,
flavonoids, alkaloids, steroids and phenylpropanoid glycosides. Thus, the oil has
strong pharmacological activity and can be used as a raw material for
aromatherapy, perfume, cosmetics, insecticides, and pharmaceuticals. The growing
demand for patchouli oil in Indonesia has not been fully met because conventional
cultivation is considered difficult to produce patchouli oil yields with good quality
and high patchouli alcohol content. In vitro cultivation in a TIS-RITA® bioreactor
can produce superior patchouli seedlings with similar characteristics, pathogen
free and stress tolerant. In this study, in vitro cultivation of patchouli was performed
in the TIS-RITA® bioreactor at a flow rate of 15.67 mL/min with the addition of 10
µM MeJA. MeJA elicitor acts as a self-defense response to induce genes involved
in the patchouli alcohol biosynthesis pathway. The purpose of this study was to
determine the effect of the addition of 10 µM MeJA elicitor on the growth and oil
yield of patchouli when grown in vitro in the TIS-RITA® bioreactor. In the results
of this study, there was an increase in growth with MeJA 10 µM treatment in the
TIS RITA® bioreactor, which included the percentage of biomass increase of
42.265 ± 4.1%, the relative growth rate of 0.025 ± 0.004 g/day, the average growth
rate of 0.031 ± 0.004 cm/day, and the percentage of shoot growth of 46.905 ± 4.2%.
Meanwhile, the growth of the control was lower. The survival rate of patchouli in
both control and treatment was 100%. Patchouli oil yield obtained by steam
distillation in MeJA 10 µM treatment was higher at 8.57 ± 0.79% (w/w) compared
to control at 9.08 ± 0.78% (w/w). Analysis of the medium conducted as an
indication of nutrient bioconversion by patchouli culture showed a decrease in pH,
conductivity and sucrose content |
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