CARBON PASTE ELECTRODE MODIFIED WITH ZNO AND MOLECULARLY IMPRINTED POLY(GLYCINE) FOR DETERMINATION OF RHODAMINE B BY VOLTAMMETRIC METHODS

• Main Author: Felicia Saputra, Evelyn
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/83368
• Institution: Institut Teknologi Bandung

Rhodamine B is a dye from the xhantene dye group that is used as a bright red dye in the textile and plastics industry. Rhodamine B is a hazardous substance and has been stipulated regulated by the Ministry of Health of the Republic of Indonesia. Therefore, the rhodamine B content in food must be further analyzed further. One of the analytical methods used to analyze rhodamine B in food is voltammetry. The voltammetry method is simple, sensitive, and selective. The working electrode that is often used in the voltammetry method is the carbon paste electrode (CPE). In this research, carbon paste electrode modified with ZnO nanoparticles and molecularly imprinted polyglycine (MIP) was developed for the determination of rhodamine B. The results show that CPE-ZnO-MIP gives a higher peak current than bare CPE. The optimum conditions for CPE-ZnO-MIP were obtained with a molar rasio of rhodamine B to glycine of 1:2 and 10 electropolimerization cycles. The optimum condition for rhodamine B measurement was obtained with square wave voltammetry in 0.1 M phosphat buffer solution at pH 7. CPE-ZnO-MIP had good repeatability and reproducibility with %RSD of 3,83% and 3,55%, respectively. The linear range of CPE-ZnO-MIP is 1 – 100 µM with a detection limit is 0,595 µM. Analysis of the scan rate shows that the electron transfer process on the electrode is controlled by diffusion. CPE-ZnO-MIP is quite selective towards rhodamine B in presence of interfering compounds commonly in food. Rhodamine B in crackers samples are analyzed by CPE-ZnO-MIP was not significantly different from measurements using UV-Vis spectrophotometer.