UTILIZATION OF AMNIOTIC MEMBRANE EXTRACT AS A CHITOSAN/POLYVINYL ALCOHOL-BASED HYDROGEL COMPOSITE FOR WOUND HEALING
The skin is the largest organ of the body, functionally playing a role in maintaining homeostasis by regulating temperature, hydration, and vitamin D synthesis, as well as serving as a barrier against external chemicals and pathogens. Damage to the skin can disrupt these functions and increase the r...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/83674 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | The skin is the largest organ of the body, functionally playing a role in maintaining homeostasis by regulating temperature, hydration, and vitamin D synthesis, as well as serving as a barrier against external chemicals and pathogens. Damage to the skin can disrupt these functions and increase the risk of other health complications. Chronic skin wounds can lead to increased pain, stress, and even death. Hydrogels are three-dimensional polymer networks that are hydrophilic. Previous research has shown that hydrogels can be developed as scaffolds for wound healing due to their potential to retain oxygen, absorb wound exudate, maintain a moist environment that supports physiological processes, and facilitate gas exchange due to their porous structure. This study focuses on analyzing the physical and biological properties of hydrogel scaffolds composed of chitosan and polyvinyl alcohol (PVA) combined with amniotic membrane extract (AME) to support the wound healing process in vitro. The fabrication of these hydrogels was carried out using the freeze-thaw method with four freezing cycles and the addition of glycerol as a cross-linker. In this study, three types of hydrogels were fabricated: chitosan-PVA (1:1), chitosan:PVA:AME 5 mg/mL (4:4:1), and chitosan:PVA:AME 8 mg/mL (4:4:1). Physical characterization of hydrogels included water contact angle, swelling, water content, and degradability. In vitro biological characterization was analyzed by measuring the cytotoxicity and proliferation of human fibroblast cell line (1BR3) using the MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide). The result showed that the hydrogels had hydrophilic surface with a contact angle of <90o, high swelling ratio values with percentages > 150%, water content below 20%, and optimal degradation capability over 14 days. These hydrogel characteristics are suitable for wound dressing in wound healing. The hydrogels were non-toxic as 1BR3 cells grown for three days had cell viability > 70% (ISO 10993-5). Additionally, there was an increase in the viability of 1BR3 cells grown on the hydrogels on days 3, 5, 7, and 10, furthermore the addition of AME to the hydrogel scaffold can enhance cell viability. Cells could grow on the hydrogels and form aggregates. Observations of cell aggregates on days 1, 2, and 5 showed an increase in the number and size of cell aggregates. It can be concluded that hydrogels composed of chitosan/polyvinyl alcohol/amniotic membrane extract are biocompatible and can support cell growth, thus having a potential for wound healing.
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