POLYCAPROLACTONE/GELATIN METHACRYLATE/CERIUM (IV) OXIDE BASED ANTIBACTERIAL MEMBRANE FOR WOUND HEALING APPLICATION

• Main Author: Adilvi, Steve
• Format: Final Project
• Language: Indonesia
• Subjects: Teknik (Rekayasa, enjinering dan kegiatan berkaitan)
• Online Access: https://digilib.itb.ac.id/gdl/view/84362
• Institution: Institut Teknologi Bandung

One of the many complications experienced by diabetes mellitus patients is diabetic foot ulcer (DFU). DFU is prone to bacterial infections and can lead to amputation. Therefore, many studies have developed methods for treating DFU, including the use of nanofibrous dressings (ND) fabricated through electrospinning. ND has a unique advantage in that its structure resembles the extracellular matrix (ECM), allowing it to stimulate the growth of new skin tissue. In this study, ND based on polycaprolactone (PCL) and gelatin methacrylate (GelMA) with the addition of cerium (IV) oxide (CeO2) nanoparticles has been developed. PCL is a commonly used biomaterial due to its biocompatibility and good mechanical properties. However, PCL is hydrophobic, making it difficult for cells to adhere. Therefore, GelMA was added to the membrane as a bioactive component to promote cell adhesion and proliferation, and reduce the hydrophobicity of the membrane. In addition, CeO2 nanoparticles were added to prevent bacterial infections due to their antibacterial properties. The test results show that the PCL/GelMA/CeO2 2% membrane has the tensile strength and elongation of 9.28 ± 1.74 MPa and 86.06 ± 28.54%, respectively which mimic the tensile properties of human skin. Additionally, the membrane has an optimal swelling ratio of 151.33 ± 5.43%, which is suitable for ND membranes. The antibacterial activity test shows that this membrane is able to kill 99.39% of S. aureus bacteria and 99.50% of E. coli bacteria. Therefore, it can be concluded that the PCL/GelMA/CeO2 2% membrane has the potential for further development as an ND membrane for treating DFU.