STABILITY ASSAY AND CONCENTRATION OPTIMIZATION OF MAGNESIUM CHLORIDE IN REACTION BUFFER AND DITHIOTHREITOL (DTT) IN STORAGE BUFFER FOR REVERSE TRANSCRIPTASE SIS7A FUSION
Reverse transcriptase (RT) is an enzyme with the ability to convert RNA chains into DNA chains, which is highly useful in molecular biology. To enhance the processivity and thermostability of RT, previous research involved engineering by fusing Sis7a with MMLV reverse transcriptase. However, to d...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/84421 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |