SCREEN-PRINTED CARBON ELECTRODE MODIFIED GOLD NANODENDRITE (SPCE-AUND) AS NON-ENZYMATIC ELECTROCHEMICAL BIOSENSOR FOR URIC ACID DETECTION

• Main Author: Ihsantia Ning Asih, Geolita
• Format: Theses
• Language: Indonesia
• Subjects: Teknologi
• Online Access: https://digilib.itb.ac.id/gdl/view/84457
• Institution: Institut Teknologi Bandung

Sensor development in the era of chemical sensors utilizes conventional analysis methods that are based on the separation of complex samples. These methods require sophisticated equipment and procedures that can hinder real-time sample analysis. Conventional techniques are starting to be displaced by the development of biosensors that can provide fast, real-time responses, have good sensitivity and selectivity, and are non-invasive. Electrochemical biosensors are one method of analysis to provide routine health monitoring facilities that are fast, inexpensive, and can be used for early detection at home. Screen-Printed Carbon Electrode (SPCE) is one type of electrode that can support point-of-care (POC) sample analysis because of its small size so that it can be integrated with portable devices. In its development, biosensor modification is needed to improve its performance in terms of selectivity, stability, sensitivity, linearity, and reproducibility. The rapid development of nanoparticle science, especially in the field of biomedicine and biosensors, plays a major role in the process of diagnosing a disease. Modification of biosensors using gold nanodendrites (AuND) has attracted much attention. Its branched morphology provides large active sites that can increase its electrocatalytic activity compared to other morphologies. The electrodeposition method of AuND synthesis is promising because it can grow gold nanodendrite structures that are easily controlled and specific to the expected area. In this study, surface modification of SPCE using gold nanodendrites (SPCE-AuND) was electrodeposited. The process was optimized to produce electrodes with the best electrochemical performance. The characteristics of the AuND successfully grown on the working electrode (WE) surface were analyzed using SEM and XRD. Then, SPCE-AuND with optimal parameters was tested for electrochemical characteristics and performance in the application of a non-enzymatic electrochemical biosensor to detect uric acid (UA). The methods used were cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The detection performance values of the biosensor in the form of limit of detection (LOD), limit of quantification (LOQ), and linear range obtained values of 0.04 mM; 0.12 mM; and 0.1-5 mM. These performance values are below the normal range of uric acid concentration in human body fluids, so SPCE-AuND has the potential to be developed as a non-enzymatic electrochemical biosensor for uric acid (UA) detection.