POTENTIAL OF COW MANURE BIODIGESTER WASTE ON PROTEIN, ASTAXANTHIN, AND PHYCOCYANIN CONTENTS IN MIXED CULTURE OF SPIRULINA PLATENSIS AND HAEMATOCOCCUS PLUVIALIS IN OPEN RACEWAY POND

• Main Author: Wicaksono Djuhadi, Benedictus
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/84627
• Institution: Institut Teknologi Bandung

Microalgae are aquatic microorganisms that have many benefits because they have high nutritional content, contain bioactive compounds, and are able to survive in extreme environmental conditions. Although they offer many benefits, industrialscale microalgae production requires high operational costs for the needs of microalgae cultivation media. Anaerobically digested dairy manure wastewater (ADDMW) is cow dung waste that has been processed in biogas production. The high nitrogen content in ADDMW makes this waste potentially used as a microalgae cultivation medium. This study aims to review the potential of ADDMW as a substitute for synthetic media for mixed cultivation of Spirulina platensis and Haematococcus pluvialis and the accumulation of protein, astaxanthin, and phycocyanin. The study was conducted with variations in the addition of ADDMW waste to the Zarrouk medium with concentrations of 0%, 50%, and 100%. Mixed culture microalgae were cultivated for 20 days at a temperature of 25±2oC, with a light intensity of 2100 lux, a photoperiod of 16:8 light:dark, and aeration of 0.5 L/minute. Microalgae samples were taken every two days to determine the growth rate of microalgae based on dry weight, determination of protein content by the Bradford method, astaxanthin content by high performance liquid chromatography (HPLC), and phycocyanin content by spectrophotometry. The results showed that the concentration of ADDMW waste of 50% was the most optimal for mixed culture microalgae cultivation with a specific growth rate of 0.087/day, protein, astaxanthin, and phycocyanin levels of 0.907±0.72%, 15.48±3.35 µg/g, and 2.255±0.97 mg/g, respectively. The results of the scaling-up process in the open raceway pond showed an increase of 4,5x in protein content and a decrease in astaxanthin and phycocyanin content of 66, and 76%, respectively. The conclusion of this study is that ADDMW waste has the potential to substitute Zaarrouk’s medium for protein production, but has not been able to substitute Zarrouk’s medium for astaxanthin and phycocyanin production