A STUDY ON THE EFFICIENCY OF FILAMENTOUS AND NON-FILAMENTOUS FUNGI IN DEGRADING TOTAL PETROLEUM HYDROCARBON (TPH) IN OILCONTAMINATED SOIL

• Main Author: Adriansyah, Fadhlurrahman
• Format: Final Project
• Language: Indonesia
• Subjects: Teknik saniter dan perkotaan; teknik perlindungan lingkungan
• Online Access: https://digilib.itb.ac.id/gdl/view/84668
• Institution: Institut Teknologi Bandung

In the exploitation, production, and transportation of petroleum, hydrocarbon pollution often occurs due to spills, accidents, and unregulated disposal. This pollution contains Total Petroleum Hydrocarbon (TPH), which includes harmful compounds such as BTEX (benzene, ethylbenzene, toluene, xylene). Mycoremediation uses fungal processes to break down contaminants into forms that are safer for the environment. In this study, several fungal species were selected, including Rhizopus oryzae, Saccharomyces cerevisiae, and Marasmius sp., as the main agents for degrading TPH in media taken from oil-contaminated soil in Minas Oil Field, Riau, due to their ability to produce ligninolytic enzymes such as LiP, MnP, and laccase. Variables considered include nutrients, pH, and moisture content, with anaerobic treatment at 35°C, both with and without bulking agent. The tested soil was loam with initial TPH of 0.5-0.7%, total phosphorus of 93.49 mg/kg, total Kjeldahl nitrogen of 1097.7 mg/kg, moisture content of 62%, and pH of 6. The results showed the highest TPH degradation by Marasmius sp. with bulking agent (91.5%), followed by R. oryzae (77.2%) and S. cerevisiae (62.5%). Without bulking agent, all three fungi showed similar results, around 57%. R. oryzae fungus achieved the highest biodegradation rate of 0.151% per week, while Marasmius sp. had the lowest at 0.0836% per week. With the addition of a bulking agent, Marasmius sp. reached the highest rate of 0.1906% per week, while S. cerevisiae and R. oryzae had the same rate of 0.1186% per week This degradation resulted in final pH of 5.5-5, moisture content of 40%-50%, total phosphorus of 46- 25 mg/kg, and total Kjeldahl nitrogen of 670-337 mg/kg.