ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT AND FRACTION OF FOUR GRADES TYPES OF BLACK TEA (CAMELLIA SINENSIS (L.) KUNTZE) AGAINST ACNE CAUSING BACTERIA

• Main Author: Gunawan, Christian
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/85199
• Institution: Institut Teknologi Bandung

Acne is the most common skin disease, with the highest percentage in adolescents and adults. In the pathogenesis of acne, the natural microflora of the skin plays an important role, as do the bacteria Cutibacterium acnes and Staphylococcus epidermidis. The use of antibiotics for acne can lead to acne resistance. The alternative for acne treatment is using natural ingredients that with antibacterial activity. Tea is one of the plants with antibacterial activity. Camellia sinensis (L.) Kuntze or tea is a plant belonging to the Theaceae family. We can classify it into four distinct classes, which are black tea, oolong tea, green tea, and white tea. This study aims to test the antibacterial activity of four different classes of black tea leaf quality against acne-causing bacteria, which are Cutibacterium acnes and Staphylococcus epidermidis.The crude drugs were extracted by maceration method using 70% ethanol as a solvent. The extracts were then subjected to preliminary tests using disc diffusion. The extract that produced the largest inhibition diameter in the disc diffusion test was fractionated using liquid-liquid extraction method. The extract and all fractions were tested for antibacterial activity against Cutibacterium acnes ATCC 11827 and Staphylococcus epidermidis ATCC 12228 bacteria using microdilution and drop test methods. Based on the results, the ethanol extract of BOPF type black tea leaves produced the largest inhibition diameter compared to the other extracts against Cutibacterium acnes and Staphylococcus epidermidis bacteria. The ethyl acetate fraction of black tea ethanol extract had the strongest antibacterial activity against Cutibacterium acnes bacteria with a MIC value of 256 ?g/mL and MIB 512 ?g/mL, while the black tea extract had the strongest antibacterial activity against Staphylococcus epidermidis bacteria with a MIC value of 256 ?g/mL.