ISOLATION OF ANTIOXIDANT COMPOUND FROM OIL AND SEED DREGS OF MALAPARI (PONGAMIA PINNATA (L.) PIERRE) BIODIESEL PRODUCTION

• Main Author: Tanuwijaya, Kathleen
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/85206
• Institution: Institut Teknologi Bandung

Free radicals are reactive compounds. Free radicals at an excessive and imbalance amount may pose as a harm towards human health. This condition is also known as oxidative stress. To overcome the harmful effects of stress oxidative, antioxidant is needed. Antioxidants are compounds capable of inhibiting free radicals. One of the plants that is known to have antioxidant activity is locally known as malapari (Pongamia pinnata (L.) Pierre). This research was aimed to isolate active antioxidant compound in malapari seed oil and its waste from the biodiesel production process. Additionally, this research was also aimed to determine the secondary metabolite compound group of the active compound. Antioxidant assay was done using DPPH assay method, both qualitatively and quantitatively. Qualitative assay was done using thin layer chromatography (TLC) bioautography. Quantitative assay was done using spectrophotometry UV-Vis method to determine % RSA (radical scavenging activity) and IC50 value. IC50 value refers to the concentration of a sample capable of inhibiting 50% free radicals. The IC50¬ value for ethanol seed waste extract were 2,187.60 ± 22.03 ?g/mL. The seed oil at concentration of 2,000 ?g/mL could only inhibit 17.92% of free radicals (DPPH). This indicated that the ethanol seed waste extract had stronger antioxidant activity compared to the seed oil. Therefore, ethanol seed waste extract was chosen to continue the isolation process. The qualitative antioxidant assay of the isolate obtained from the ethanol seed waste extract showed a yellow spot against purple background. This implied that the isolate was an antioxidant. The isolate with concentration of 116.66 ?g/mL exhibited % RSA of 0.29 ± 0.19% and was identified as a phenolic acid compound.