ANTI-PEPTIC ULCER ACTIVITY TEST OF BASIL LEAF NANOSUSPENSION (OCIMUM AMERICANUM L.) IN MALE WISTAR RATS
Peptic ulcer is a disease of the gastrointestinal system characterized by lesions in the mucosal layer, resulting from an imbalance between aggressive and defensive factors in the stomach. The treatment of peptic ulcers with traditional medicine derived from plants has been empirically used by co...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/85359 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Peptic ulcer is a disease of the gastrointestinal system characterized by lesions in the mucosal layer,
resulting from an imbalance between aggressive and defensive factors in the stomach. The treatment
of peptic ulcers with traditional medicine derived from plants has been empirically used by
communities, with basil leaves being one notable example due to their high antioxidant content.
While the ethanol extract of basil leaves has been proven effective in treating peptic ulcers, no studies
have yet examined the use of basil in the form of nanosuspension. Nanosuspension formulations are
known to enhance solubility and bioavailability. This study aims to determine the anti-peptic ulcer
activity of basil leaf extract formulated into nanosuspensions. The study used male Wistar rats, which
were divided into six groups: the normal group (CMC-Na 0.5%), negative control (96% ethanol),
positive control (sucralfate 200 mg/kg BW), basil extract group (100 mg/kg BW), basil nanosuspension
group (100 mg/kg BW), and a base group (saccharide & arginine). Observations were made over 6
days, with all groups except the normal group being induced with 96% ethanol at a dose of 1 mL/200
g BW on days 1 and 6, and 0.5 mL/200 g BW on days 2 through 5. The test animals were sacrificed one
hour after induction on the 6th day. The results of the anti-ulcer activity test showed that the basil
nanosuspension group had the lowest gastric ulcer index of 5.92, with the highest ulcer inhibition
percentage of 63.39%. The pH and gastric fluid weight were 5.10 ± 0.37 and 248.96 ± 91.302,
respectively, with histological analysis showing similarities to the normal group. The results of the
antioxidant test for the basil nanosuspension demonstrated a decrease in MDA levels and an increase
in CUPRAC levels compared to the negative control, indicating that basil nanosuspension possesses
high antioxidant activity. Based on the overall results, basil nanosuspension at a dose of 100 mg/kg
BW exhibited the most effective anti-peptic ulcer activity across all parameters.
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