THE POTENCY OF A DIATOM CYCLOTELLA STRIATA STRAIN TBI TO DECREASE FE(III) ION CONTENT IN CULTURE MEDIA
Diatoms are microalgae commonly found in nature. Diatoms are capable to live in waters exposed to heavy metals. Several heavy metal’s ions are known to be nutrients important for the survival of diatoms, one of which is the Fe(III) ion. Exploring the potency of diatoms in surviving heavy metal’s wat...
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| Format: | Final Project |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/85743 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Diatoms are microalgae commonly found in nature. Diatoms are capable to live in waters exposed to heavy metals. Several heavy metal’s ions are known to be nutrients important for the survival of diatoms, one of which is the Fe(III) ion. Exploring the potency of diatoms in surviving heavy metal’s water could be a strategic step to decrease the Fe(III) ion content in waters. For this reason, it is necessary to conduct initial studies on a smaller scale, especially on diatom culture media. This study aims to explore the potential of the diatom Cyclotella striata TBI strain to decrease the Fe(III) ion content in the culture media. The sources of Fe(III) ions used in this experiment were FeEDTA and FeCl3.6H2O. This study was initiated with the cultivation of C. striata on F/2 media and F/2 media containing Fe(III) ion contaminants of 2,00 and 4,00 mg/L from FeEDTA and FeCl3.6H2O. Then, the growth studies of C. striata was carried out daily using a light microscope and OD676 measurements. Changes in Fe(III) ion levels in the media were determined using AAS. Furthermore, physical analysis of DO (Dissolved Oxygen) and pH measurements were carried out to complete the analysis of C. striata growth. The results of this study indicate that C. striata grown in F/2 media containing 2,00 mg/L Fe(III) ion contaminants from FeCl3.6H2O grew more than C. striata in F/2 media without contaminants. In both conditions, there was a decrease in Fe(III) ion levels in the growth media. The largest decrease in Fe(III) levels was observed in media containing Fe(III) contaminants, which was 3,80 mg/L. DO analysis showed that along with the increasing number of C. striata, the DO value decreased. Conversely, pH analysis showed that along with the increasing number of C. striata, the pH of the media increased. Interestingly, the growth of C. striata was inhibited in the media containing 4,00 mg/L Fe(III) from FeCl3.6H2O and in media with Fe(III) from FeEDTA. This study shows that C. striata can decrease Fe(III) levels in the media, however, this ability depends on the concentration and source of Fe(III) ions |
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