ISOLATION AND ACTIVITY TEST OF ACTIVE COMPOUNDS TO INHIBIT THE GROWTH OF ACNE-CAUSING BACTERIA FROM JAVANESE TURMERIC RHIZOME (CURCUMA XANTHORRHIZA ROXB.)

Temulawak (Curcuma xanthorrhiza Roxb.) has many benefits, one of which is that it can treat acne. Therefore, based on its activities, temulawak rhizomes have great potential to be developed for the treatment of acne and the development of anti-acne cosmetic products. In this research, active compoun...

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Bibliographic Details
Main Author: Ainun Hambali, Isqi
Format: Final Project
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/86295
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Institution: Institut Teknologi Bandung
Language: Indonesia
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Summary:Temulawak (Curcuma xanthorrhiza Roxb.) has many benefits, one of which is that it can treat acne. Therefore, based on its activities, temulawak rhizomes have great potential to be developed for the treatment of acne and the development of anti-acne cosmetic products. In this research, active compounds were isolated that were able to inhibit the growth of acne-causing bacteria from temulawak rhizomes and tested the antibacterial activity of Temulawak rhizomes. This research was carried out through a series of stages, namely simplicia characterization, extraction, phytochemical screening of simplicia and extracts, fractionation, subfractionation, isolation and purification, and purity testing. These stages follow the instructions for the highest antibacterial activity of the tested samples to proceed to the next stage. The process begins with extraction of Temulawak simplicia using the maceration method using 3 different types of solvents, namely ethanol, ethyl acetate, and n-hexane. These extracts were tested for antibacterial activity using the microdilution method to measure the minimum inhibitory concentration (MIC). The selected extract which had the greatest antibacterial activity was fractionated using the vacuum liquid chromatography method using n-hexane - ethyl acetate solvent with increasing polarity and continued testing of the antibacterial activity of the fraction obtained using the microdilution method. The selected fraction which has the greatest antibacterial activity is continued to the subfractionation process using the vacuum liquid chromatography method so that 5 types of combined subfractions are produced and then continued with testing the antibacterial activity of the subfractions using the microdilution method. The selected subfraction which has the greatest antibacterial activity is then continued to the isolation process using radial chromatography. Furthermore, antibacterial activity testing of candidate isolates was carried out using the microdilution method and resulted in sample isolate B25 (origin of candidate isolate 11) which had the highest antibacterial activity by producing an MIC of 8 ?g/ml (strong category) on Propionibacterium acnes bacteria and producing an MIC of 16 ?g/ml (strong category) on Staphylococcus aureus bacteria.