EVALUATION OF THE INFLUENCE OF SALICYLIC ACID ELICITATION ON THE PRODUCTION OF DIELS-ALDER ADDUCT COMPOUNDS IN MORUS CATHAYANA ROOT CULTURES
Morus cathayana is one of the less-studied species within the Morus genus. A notable group of compounds found in this plant are the Diels-Alder adducts, which exhibit diverse pharmacological activities, including antibacterial and cytotoxic effects. However, further investigation of these compoun...
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| Format: | Theses |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/86907 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Morus cathayana is one of the less-studied species within the Morus genus. A notable group of
compounds found in this plant are the Diels-Alder adducts, which exhibit diverse
pharmacological activities, including antibacterial and cytotoxic effects. However, further
investigation of these compounds is hindered by their scarcity in nature. Tissue culture
techniques provide a viable solution to this limitation, offering advantages such as efficient
land use, mass propagation, and genetic consistency. Nevertheless, secondary metabolite
biosynthesis in tissue culture is often suboptimal and differs from that in the parent plant,
necessitating optimization. One promising optimization method is elicitation, which leverages
plant stress responses to enhance secondary metabolite production. This study aims to evaluate
the effect of salicylic acid elicitation on the production of Diels-Alder adducts using
multivariate analysis based on HPLC. M. cathayana root cultures were cultivated in
Murashige and Skoog media and elicited with salicylic acid at varying elicitation times. HPLC
analysis detected 52 metabolite signals in M. cathayana root extracts. Four compounds were
quantified, five compounds were qualitatively identified, and 43 remained unidentified.
Multivariate data analysis revealed that elicitation time had varying effects on the biosynthesis
of nine standard compounds. Antibacterial assays against E. faecalis showed an inhibition
zone of 11 mm for both the control and elicited samples. For P. mirabilis, the inhibition zones
were 10.8 mm for the control and 12 mm for elicited samples. Neither the control nor the
elicited samples inhibited V. cholerae or S. aureus. Cytotoxicity test against MCF-7 cells
showed that elicited samples showed a stronger cytotoxic effect than the control with an IC50
value of 4.8 ± 1.36 µg/mL.
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