CARBON PASTE ELECTRODE MODIFIED WITH ZNO NANOPARTICLE AND POLYPYROLE FOR VOLTAMMETRIC ANALYSIS OF DICLOFENAC

• Main Author: Wulandari, Selly
• Format: Final Project
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/87263
• Institution: Institut Teknologi Bandung

Diclofenac is a nonsteroidal anti-inflammatory drug (NSAID) with analgesic, anti inflammatory, and antipretic effects. Diclofenac is one of the NSAID drugs that is very commonly used so that the waste of use is more than other NSAID drugs. The water-soluble nature and the lack of a biodegradation system cause diclofenac to receive special attention because this drug waste has begun to pollute the environment, especially in waters that can cause damage to ecosystems and marine organisms. Therefore, it is necessary to measure diclofenac levels in the surrounding environment so that pollution can be addressed immediately. This study aims to modify carbon paste electrodes (CPE) using ZnO nanoparticles and molecular imprinted polypyrrole (MIP) for voltammetric analysis of diclofenac. A good EPK modification showed an increase in the peak current measured, indicating an increase in measurement sensitivity in diclofenac analyte solution. The modification of EPK with ZnO was performed using two methods: the drop-casting method and the bulk phase method. The ZnO-modified EPK was then electropolymerized using the MIP method to form a polymer layer on the surface. The performance of the modified electrode was optimized by evaluating MIP composition, electropolymerization cycles, pH, and voltammetry techniques to achieve optimal measurement results. The optimized EPK-ZnO MIP was tested for performance based on repeatability, reproducibility, scan rate, linearity, and interfering compounds. Based on this study, the optimum conditions for diclofenac measurement using ZnO and polypyrrole modified EPK were obtained at 8% (w/w) ZnO composition of concentration at 1:3 MIP composition in PBS pH 7 0.1 M with 10 cycles of electropolymerization using square wave voltammetry (SWV) technique. Electrode performance tests showed EPK-ZnO-MIP has repeatability and reproducibility with %RSD of 6.014% and 2.648% which indicates CPE has good precision and reliability. Scan rate analysis showed diffusion controlled electron transfer. The linear region of diclofenac measurement was in the range of 10-60 µM and the of detection was 2.313 µM. Analysis of drug samples by voltammetry and HPLC methods resulted in %recovery values of 98.18% and 96.79%. These values indicate that the voltammetric method with CPE modified with ZnO nanoparticles and polypyrrole has high measurement sensitivity.