#TITLE_ALTERNATIVE#

• Main Author: , Mulyadi
• Format: Dissertations
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/8746
• Institution: Institut Teknologi Bandung

Abstact: <br /> <br /> <br /> The aim of this work is to investigate the effects of benzo(a)pyrene (BP) and 7,12-dimethylbenz(a)anthracene (DMBA) on Turbellaria. It is Down that Turbellaria belongs to the simplest Protostoma. These animals can be succesfully cultivated in our laboratory by using a suitable growth media at 250C, while vigorous shaking <br /> <br /> <br /> must be avoided. In the course of further investigation it was found that BP as well as DMBA was not able to induce morphological changes in Turbellaria. On the other hand 7-hydroxymethyl 12-methylbenz(a)anthracene, which is known as an ultimate carcinogen, appeared to influence the morphology of the same experimental animals. It is known that the latter substance can be formed from DMBA in animal tissues whereby cytochrome P-450 plays an important role. Further experiments revealed that this cytochrome was absent in Turbellaria, so this can explain why BP and DMBA were not active. <br /> <br /> <br /> Further it must be noted that the morphological defects disappeared within 11 days. This might be due to the fact that Turbellaria has a remarkable ability to regenerate itselves. This fact seems to be closely related to the existence of an efficient repair mechanism for DNA. <br /> <br /> <br /> However, in spite of the absence of cytocbrome P-450, BP as well as DMBA was able to disturb protein and glycogen synthesis.