EFFECTS OF NUTRITION ON THE PRODUCTION OF UNSATURATED FATTY ACIDS AND LIPIDS FROM CHLORELLA PYRENOIDOSA

• Main Author: Rasyid Assidiqi, Azmi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/87768
• Institution: Institut Teknologi Bandung

The dairy industry is one of many industries that produce large amounts of liquid waste, with milk processing generating 1.1-6.8 L for every litre of milk produced while 887 million tons of milk are produced in 2021 alone. Microalgae are able to process dairy wastewater, absorbing the dissolved carbon, nitrogen, and phosphor, while producing lipids and fatty acids that can be processed to create biodiesel. This research aims to study the effects of using dairy wastewater as a substrate and the addition of glycerol as a source of carbon towards the production of lipids and fatty acids by the microalgae Chlorella pyrenoidosa. The independent variables for this research are the concentration of dairy wastewater (0%, 10%, 25%, and 50%) and the addition of glycerol (0, 5, 10, and 15 g/L) in the growth medium. The results show that the addition of glycerol and dairy wastewater increases the specific growth rate, biomass productivity, and lipid production until a saturation point is reached. The highest specific growth rate (0,228 day-1) is achieved using 25% dairy wastewater and 5 g/L of glycerol. Meanwhile, highest biomass yield (0,353 g/L) is achieved using 50% dairy wastewater and 10 g/L of glycerol. The medium with 25% dairy wastewater and 10 g/L of glycerol is optimal for lipid production (37.0% cell dry weight). High nutrient concentration increases the length of the lipid carbon chains, although the overall fatty acid composition is predominantly C10 or capric acid.