DEVELOPMENT OF MITRAGYNINE ENRICHMENT ISOLATION METHOD USING MACROPOROUS RESINS PAD610 AND PAD900

• Main Author: Febriandi, Rizal
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/87981
• Institution: Institut Teknologi Bandung

Kratom (Mitragyna speciosa) contains mitragynine in high concentration, which has pharmacological activity as an antinociceptive, sedative, antimicrobial agent, and can help relieve alcohol dependence. This study aims to develop a method for isolating mitragynine compounds using macroporous resins that is safe, environmentally friendly, cost-effective, and efficient to enrich the acquisition of mitragynine compounds using macroporous resins PAD900 and PAD610. Extraction was carried out using the maceration method with 70% ethanol as a solvent. Evaluation was carried out on the performance and efficiency of macroporous resins in enriching mitragynine, as well as the adsorption and desorption properties of the resin, using UPLC-MSMS. The results showed that the adsorption and desorption processes of the resin on mitragynine in the extract followed a second-order model, indicating that mitragynine adsorption was influenced by other compounds. The isothermal adsorption test showed good balance with the Freundlich model for the PAD610 (R2 = 0.9973) and PAD900 (R2 = 0.9905) resin types. Under optimal conditions, the process of enrichment of mitragynine compound isolation using PAD610 and PAD900 macroporous resins is able to increase the mitragynine content as evidenced by the concentration of mitragynine compounds in the extract of 2.56% to 7.58% in PAD610 and 18.83% in PAD900 with a good flow rate shown at a flow rate of 3 BV/hour and of the two resins, PAD900 resin has the ability to adsorb mitragynine compounds better than PAD610 resin.