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Maternally inherited mitochondrial DNA has been applied for medical purpose, such as for diagnose Diabetes Mellitus (DM). It has been reported that Maternallu Inherited Diabetes and Deafness has a correlation with mitochondrial DNA mutation, which is a A3243G mutation on tRNALeu gene. However, there...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/9155 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Maternally inherited mitochondrial DNA has been applied for medical purpose, such as for diagnose Diabetes Mellitus (DM). It has been reported that Maternallu Inherited Diabetes and Deafness has a correlation with mitochondrial DNA mutation, which is a A3243G mutation on tRNALeu gene. However, there is no information whether mitochondrial DNA sample in Biochemistry Laboratorium at the Division of Biochemistry, ITB posses its mutation. Samples were obtained from mouth epithelial cell, then DNA fragment were amplified using Polymerase Chain Reaction method, followed by an agarose gel electrophoresis, and sequencing mtDNA of samples, finally the results were analyzed by using SeqmanTM program. Visualization of the amplified mtDNA showed one band of 1,8 kb in size. The results indicate that samples has A3606G mutation, instead of A3243G. Based on these results, it can be conclude that the mtDNA of this diabetes patient has no correlation with A3243G mutation. However, as has been reported recently, this A3606G mutation might be associated to certain diabetes patient, including the one in this research. |
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