#TITLE_ALTERNATIVE#

• Main Author: Hasibuan (NIM 253 96 003), Saberina
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/9189
• Institution: Institut Teknologi Bandung

Abstract : <br /> <br /> <br /> The standard method for bioassay to determine lethal concentrations are usually done by using the related hazardous substances in its procedure, hence, producing laboratory hazardous wastes. This study was intended (i) to evaluate gills histologicly when exposed to Cadmium in 25, 50, and 75 lethal concentrations, for 96 hours and observation was continued to 240 hours instead with carp (Cyprus carpio L) as experimental animal to measure exposure concentration, and (ii) colored by BTAN-beta. Damage intensity and the extent of color absorption were examined to see if it can be used to measure exposure concentration. Besides (iii) measurement of metallothionein was done to see if it can be used Cd indicator of fresh water pollution. Histopatological observations of gill structure was done by Hematoxylin and Eosin staining, lustochemistry was done by BTAN-beta staining and MTN was determined by gel filtration chromatography method and molecule weight by SDSPAGE method. Four microeecosystems (aquaria) with ten fishes in each were used in this experiment One aquarium functioned as control, and the others as the exposure condition to Cd in the concentration of LCn, LC50 and LC,5. Gills were taken out after 96 and 240 hours, divided into two portions. A portion was to be colored with HE and the other for BTAN-beta staining, while the determination of MTN was done by taking out carp liver maintained in fresh water and were named non-Cd-intoxicated or untreated animals. The liver supernatant divided into two equal parts. One part considered as control and the second part was contaminated with Cd 500 ppm. Histology preparations were made and examined for damage and color absorptions. Direct relationship between exposure time and damage intensity of carp gill was observed. Histopatological damage showed symptom of hyperplasia, hypertrophy, oedema, degeneration and necrosis at secondary and primary lamellae, as well as infarct hemorrhages either within the arches or primary lamellae; the symptoms were arranged into 6th degree damage categories. The results showed as follows : <br /> <br /> <br /> <br /> (TABLE)) <br /> <br /> <br /> <br /> Measurement of MTN concentration showed that ratio of two was treated between MTN-1 to control (MTN-I ), whereas MTN-2 increased four times in the treated sample. The molecular weight measured by SDS-PAGE was close to about 10000 daltons. <br />