STUDI PENDAHULUAN AMOBILISASI ENZIM LIPASE DARI PSEUDOMONAS COCOVENENANS DENGAN POLIAKRILAMIDA

• Main Author: Azmi, Johni
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/930
• Institution: Institut Teknologi Bandung

According to previous studies,lipase isolated from Pseudcmcras coccvenerars like other lipases is an S-H enzyme. This study concerns about the characteristic of the enzyme when immobilized with polyacrylamide and about its stability. The enzymes was produced by seeding Pseudomonas cocouenenans on Czapex Dox growth medium. The cells were than destructed by sonication. Enzyme activity was <br /> measured by the number of enzyme unit producing fatty acid equivalent to the amount of ml of 0.5 N NaOH, at a specified condition. The specific activity is defined as the number of unit activity per mgr of protein enzyme produced according to Lowry. The optimum pH of lipase was obseved at pH 8, 37oC, cystein of activation at 0.05 M and specific activity was 1,48. The same amount of, immobilized enzymes possessed a pH optimum 7,6 at 370C, cystein activation at 0.05 M and the specific activity of 2.3581• The experiment was made using the batch fermentation technique for 2 hours. Using column with 1/80 second/drop in the continuous process, it was found that immobilized lipase was stable within 32 hours with a small loss of activity.