PENGARUH INSEKTISIDA SEVIN 85 S TERHADAP PERKEMBANGAN EMBRIO DAN LARVA KODOK (BUFO MELANOSTICTUS)

• Main Author: Sagi, Memmed
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/937
• Institution: Institut Teknologi Bandung

Effect of insecticide Sevin 85 S on develoument of toad embryos and larvae ( Bufo melanostictus). Studies on the developmental abnormalities were carried out on toad embryos and, larvae( Bufo melanostictus) treated with Sevin 85 S. The exposure were done at the zygote stage to hatching, gastrula stage to hatching, neurula stage to hatching, tail/gill.bud stage to hatching, early gastrula to late gastrula, limb bud stage to pedal shaped limb, tail/gill bud stage to completes ; gill growth and hatching to pedal shaped limb. Embryos were exposed with 10, 15, 20 and 30 ppm Sevin 85 S. Observations on the hatching stage revealed that total percentage of abnormalities were significant (F.95) in the 10 ppm and highly significant (F.99) in the 15, 20 and 30 ppm. The ,percentage of abnormal tails were significant in the 10 and 15 ppm and highly significant in tree 20 and 30 ppm concentrations. Observation after the treatment to closed gill stage showed that the total percentage of abnormalities were still higherly significant in the 15, 20 and 30 ppm,:.concentration,while tail abnormalities were not significant. Statistical test using the Duncan\'s method proved that the greatest difference in the percentage of abnormalities was between control and the treatment during the neurula stage to hatching orthe treatment of tail/gill bud stage to hatching. Induced abnormalities were exencephaly, stunted growth, edema and cyclopia. The length of the larvae showed significant differences between control and treated with 15, 20 and 30 ppm, but they were not significant among the treatments. Histological structure of exencephalic larvae showed that the forebrain was exposed and head asymmetrical. Although the larvae were exposed in Sevin 85 S during the developing limb stage, no limb malformation were induced. GLC analysis of Sevin residue from the exposure of neurula. stage to hatching were 25,6 ppm (87,3 %) and. 0,8 ppm. (2,6 % .).at the closed gill stage. It was concluded that Sevin 85 S caused various developmental abnormalities and\\\' hampered growth of toad embryos and larvae.