#TITLE_ALTERNATIVE#

• Main Author: MULYATI, YETTY
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/9669
• Institution: Institut Teknologi Bandung

Penicillin can be hydrolysed by penicillin acylase into 6-aminopenicillanic acid (6-APA) and a carboxylic acid. Escherichia coli can produce this enzyme as intracellular enzyme. This investigation utilized E. coli B104 which was maintened in fermentation medium according to Morita and Iwata at pH 7.0. Phenilacetic acid (AFA) and benzilpenicillin (BP) were added into the medium as inducers, to enhance penicillin acylase production. Fermentation was carried out under aerobic condition, at 25oC for 30 hours. The cells were harvested by centrifugation followed by disruption at 0-4oC using a sonicator. The enzyme activity was tested by the colorimetric method of Kornfeld. The measurement was based on the color development after reacting with Erlich reagent. The obtained penicillin acylase, was tested for its specific activity. Benzilpenicillin was used throughout as the substrate. To enhance the production of penicillin acylase, the inductive effects of phenil acetic acid and benzilpenicillin were studied by adding into the fermentation medium 0%; 0,1%; 0,2%; 0,3%; 0,4% (b/v)AFA and 0,0025%; 0,0050%; 0,01 %; 0, 015% (b/v)BP respectively. The enzyme activity was testedc the bacteria had been incubated for 10, 20 and 30 hours respectively. The results showed, that crude penicillin acylase with the highest specific activity was obtained after 30 hours of incubation at a final concentration of 0,2% AFA and 0,0025% BP. The observed specific activities were 18,530 and 6,620 unit/mg/10 min. respectively.