DETERMINATION OF ALPHA TOCOPHEROL IN RICE BRAN OIL WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

• Main Author: JALU BRAMANTYA , ANDREW
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/9929
• Institution: Institut Teknologi Bandung

Rice bran oil is extracted from rice bran. It has high amount of antioxidant agent. The research of rice bran oil is needed to be develop so it can be used for food supplement. The purpose of this research is to analyze the amount of a-tocopherol that is one of the antioxidant agent in rice bran oil. The a-tocopherol is identified an quantified by High Performance Liquid Cromatography (HPLC) method with optimum condition as followed: mobile phase of methanol : asetonitril (4:1); isocratic elusion; Octadecyl Silane (ODS) column; flow rate 0.8 ml/minutes; spectrophotometric detector at wave length of 292nm and a-tocopherol was eluted in 5.10 minutes. Linearity test of calibration curve yielded a validation data of correlation coefficient (r) = 0.999 and variation coefficient of line regression (V0x) = 1.69%. Percent recovered test result was 96.87% at concentration level of 6.97 ug/ml. The method gave Relative Standar Deviation (RSD) respectively 1.94% at concentration level of 10 ug/ml with limit of detection and limit of quantization of 2.53 and 7.6 ug/ml. The A-tocopherol amount in rice bran oil is 1.18+0.01 mg/g. <br />