#TITLE_ALTERNATIVE#

• Main Author: DEWI SELFITRI (NIM 10704053), ANGGRAHAENI
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/9951
• Institution: Institut Teknologi Bandung

Pegagan (Centella asiatica (L.) Urban) is an important medicinal plant and it has been used to prepare herbal medicines. The compounds that are responsible for the activities are triterpenoids such as asiaticoside. Asiaticoside is also important for marker of standardization of C.asiatica. Low content and unstandardized of asiaticoside is a boottleneck for development of C. asiatica. Aim of this research was to produce higher quantity of asiaticoside from elicited and genetically transformed plant than wild type. Elicitation and genetic transformation were done in callus of C. asiatica. Callus of C. asiatica grew in Murashige-Skoog medium supplemented with 0.3% sucrose, 0.8% agar, 1 mg/L NAA as auksin, and 1 mg/L BAP as cytokinin. The elicitors were pectin in various concentrations ( 0.05, 0.1, 0.2% w/v), while genetic transformation was done by Agrobacterium rhizogenes ATCC 15834. Wild type, callus, elicited callus, transformed callus, and their control for analysis were extracted by methanol:water (9:1), then the extract were dried and redissolved. Analysis was done by thin layer chromatography (TLC) and spectrophotodensitometry. TLC Band of asiaticoside from genetic transformation callus was the most intense over all. While band of asiaticoside from elicited callus was more intense than wild type, but band from elicited callus with various concentration of pectin cannot be compared using TLC. Thin layer spectrophotodensitometry analysis of transformed callus had the most wide area under curve. Area from elicited callus was smaller than control and other callus, while wild type could not be detected. The conclusion is genetic transformation increased asiaticoside production, while pectin elicitation decreased asiaticoside production from callus but still higher than wild type. <br />