Uji Penetapan Stabilitas Retention Time Megestrole acetate Dalam Eluent Mobile Phase Menggunakan High Performmance Liquid Chromatography

• Main Authors: Siti Chusnul Cholifah, -, Mochammad Lazuardi, -, Dadik Rahardjo, -, Lilik Maslachah, -, Mohammad Sukmanadi, -, Rochmah Kurnijasanti, -
• Format: Article PeerReviewed
• Language: Indonesian; English; Indonesian
• Published: Departemen Kedokteran Dasar Veteriner Fakultas Kedokteran Hewan Universitas Airlangga 2020
• Subjects: SF910 Other diseases and conditions
• Online Access: https://repository.unair.ac.id/100758/1/Bukti%20-%20Uji%20Penetapan%20Stabilitas%20....pdf; https://repository.unair.ac.id/100758/6/Peer%20Review%20Uji%20Penetapan%20Stabilitas%20Retention%20Time%20Megestrole.pdf; https://repository.unair.ac.id/100758/3/Turnitin%2012%25_UJI%20PENETAPAN%20STABILITAS%20RETENTION%20TIME%20Megestrole%20acetate%20DALAM%20ELUENT%20MOBILE%20PHASE%20MENGGUNAKAN%20HIGH%20PERFORMANCE%20LIQUID%20CHROMATOGRAPHY.pdf; https://repository.unair.ac.id/100758/; https://e-journal.unair.ac.id/JBMV
• Institution: Universitas Airlangga

The aim of this research was to determine the level of stability of Megestrole acetate-retention time in storage period for six, eight and 12 hours using High Performance Liquid Chromatography (HPLC). The research method used posttest-only control group design by using three treatments and six repetitions. The three repetitions consist into six hours, eight hours and 12 hours. The data were obtained analyzed by Summery Independent T-Test with SPSS 24 for windows. The result showed six hours retention time of Megestrole acetate is stable and eight hours treatment and 12 hours treatment are not stable there is one unstable point of 12 hours treatment that indicates the substance is break down. Based on those result, it could be concluded that the storage time of Megestrole acetate in Eluent Mobile Phase began to show unstable at eight hours of storage Keywords stability, Megestrole acetate, retention time, High Performance Liquid Chromatography (HPLC)