Transient Receptor Potential Cation Channel Subfamily V Member-1 and Matrix Metalloproteinase-8 Expression of Capsaicin Administration in Periodontitis

• Main Authors: Jenny Sunariani, -, Titien Suhartini, -, Latief Mooduto, -, Gadis Meinarsari, -, Ari Triwardhani, -, Yuliati Yuliati, -
• Format: Article PeerReviewed
• Language: English; English; Indonesian; Indonesian; English; English
• Published: 2020
• Subjects: R Medicine; RK Dentistry
• Online Access: https://repository.unair.ac.id/102096/1/Transient%20Receptor%20Potential%20Cation.pdf; https://repository.unair.ac.id/102096/2/9.%20Transient%20Receptor%20Potential%20Cation%20Channel%20Subfamily%20V%20Member-1%20and%20Matrix%20Metalloproteinase-8%20Expression%20of%20Capsaicin%20Administration%20in%20Periodontitis.pdf; https://repository.unair.ac.id/102096/3/Dr.Ari%209.pdf; https://repository.unair.ac.id/102096/6/9.%20Transient%20Receptor.pdf; https://repository.unair.ac.id/102096/8/9.Transient.pdf; https://repository.unair.ac.id/102096/9/9.Kualitas%20Karil.pdf; https://repository.unair.ac.id/102096/
• Institution: Universitas Airlangga

The aim of this study is to prove the relationship between Transient Receptor Potential Cation Channel Subfamily V Member-1 (TRPV-1) and Matrix Metalloproteinase (MMP-8) expression in periondotitis after capsaicin administered. Twenty-one post-weaned male Wistar rats (Rattus novergius) with approximatelly 8-12 week-year-old were randomly divided into three groups (n=7). The negative control group (K0) and the positive control group (K1) were administered only with Aggregatibacter actinomycetemcomitans (A.a) bacteria serotype b to the cervical portion of their maxillary first molars. The treatment group (K2) was treated with both A.a bacteria serotype b to the cervical portion of their maxillary first molars and cayenne pepper extract at a dose of 0.0912 mg/kg/day. The subjects were anesthetized with thionembutal to obtain a sample of gingival tissue. A semi-serial thinness 4 μm by microtome, and calculate the number of cells expressing TRPV-1 and MMP-8 by immunohistochemical staining. The result of which confirmed the TRPV-1 count (K0=33.0150 ± 7.10; K1=37.63 ± 110.60; K2=43.062 ± 14.98) and MMP-8 expression (K0=43.6167 ± 14.57; K1=36.75 ± 22.53; K2=28.83 ±18.45) of the K0 group to be significantly higher than that of the K1 and K2 groups (p<0.05), while there was no significant difference in the TRPV1 andMMP-8 expression between the K0 and K1 groups (p>0.05). The TRPV-1 stimulates the lowest level of MMP-8 in rats periodontitis with capsaicin administered.