Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae
Aim: Scabies is one of the most important diseases in goats and caused by a complex hypersensitivity process that involves both humoral and cell-mediated immune responses. This phenomenon shows that the variety of Sarcoptes scabiei has different characteristics of specific antigenic protein or diffe...
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Veterinary World
2018
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id-langga.1025462022-05-11T02:50:22Z https://repository.unair.ac.id/102546/ Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae Nunuk Dyah Retno Lastuti, - Wiwik Misaco Yuniarti, - Poedji Hastutiek, - Lucia Tri Suwanti, - Dony Chrismanto, - SF600-1100 Veterinary medicine SF910 Other diseases and conditions Aim: Scabies is one of the most important diseases in goats and caused by a complex hypersensitivity process that involves both humoral and cell-mediated immune responses. This phenomenon shows that the variety of Sarcoptes scabiei has different characteristics of specific antigenic protein or different immune-dominant. This research aims to detect the humoral and cellular immune response of rabbits which were immunized with the protein of S. scabiei var. caprae. Materials and Methods: This research was done as follows, identification and collection of Sarcoptes scabiei var. caprae from goat infected with scabies, separation of protein antigen from S. scabiei mites with ultrasonic sonicator, measurement of protein content with spectrophotometry, rabbit injection with 500 μg dose of antigen protein which was repeated 5 times (5x booster) every 2 weeks. Measurement of IgG titer using indirect ELISA, whereas to detect the expression of cellular immune response (TLR-9, CD4, and CD8) using Direct Immunofluorescence assay. Results: Based on the statistical analysis, it showed that there was a significant enhancement (p<0.05) for optical density value or antibody titer and cellular immune response was shown by TLR-9, CD4, and CD8 expression in rabbit T lymphocytes which appear yellow to green fluorescent color using fluorescence microscope. The amount of fluorescence T lymphocytes showed a significant difference (p<0.05) between the control and various boosters. Conclusion: Antigenic protein of S. scabiei var. caprae contains ligands, which are involved in the pathogen-associated molecular pattern that has an ability to induce humoral and cellular immune response in rabbit. Specifically, that TLR-9 is not only involved in innate immunity but also in adaptive immunity and can be used as alternative adjuvant development research. Keywords: antigenic protein, CD4, CD8, immunoglobulin G, Sarcoptes scabiei var. caprae, toll-like receptor-9 Veterinary World 2018-06 Article PeerReviewed text en https://repository.unair.ac.id/102546/3/Bukti%20C%2003%20Humoral%20and%20celluler%20immune.pdf text en https://repository.unair.ac.id/102546/6/C03_Peer_Review_Lucia_Tri_Suwanti.pdf text en https://repository.unair.ac.id/102546/1/Turnitin%20C%2003.%20Humoral%20and%20cellular%20immune%20response%20....pdf Nunuk Dyah Retno Lastuti, - and Wiwik Misaco Yuniarti, - and Poedji Hastutiek, - and Lucia Tri Suwanti, - and Dony Chrismanto, - (2018) Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae. Veterinary World, 11 (6). pp. 819-823. ISSN 0972-8988, EISSN: 2231-0916 |
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SF600-1100 Veterinary medicine SF910 Other diseases and conditions Nunuk Dyah Retno Lastuti, - Wiwik Misaco Yuniarti, - Poedji Hastutiek, - Lucia Tri Suwanti, - Dony Chrismanto, - Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
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Aim: Scabies is one of the most important diseases in goats and caused by a complex hypersensitivity process that involves both humoral and cell-mediated immune responses. This phenomenon shows that the variety of Sarcoptes scabiei has different characteristics of specific antigenic protein or different immune-dominant. This research aims to detect the humoral and cellular immune response of rabbits which were immunized with the protein of S. scabiei var. caprae.
Materials and Methods: This research was done as follows, identification and collection of Sarcoptes scabiei var. caprae from goat infected with scabies, separation of protein antigen from S. scabiei mites with ultrasonic sonicator, measurement of protein content with spectrophotometry, rabbit injection with 500 μg dose of antigen protein which was repeated 5 times (5x booster) every 2 weeks. Measurement of IgG titer using indirect ELISA, whereas to detect the expression of cellular immune response (TLR-9, CD4, and CD8) using Direct Immunofluorescence assay.
Results: Based on the statistical analysis, it showed that there was a significant enhancement (p<0.05) for optical density value or antibody titer and cellular immune response was shown by TLR-9, CD4, and CD8 expression in rabbit T lymphocytes which appear yellow to green fluorescent color using fluorescence microscope. The amount of fluorescence T lymphocytes showed a significant difference (p<0.05) between the control and various boosters.
Conclusion: Antigenic protein of S. scabiei var. caprae contains ligands, which are involved in the pathogen-associated molecular pattern that has an ability to induce humoral and cellular immune response in rabbit. Specifically, that TLR-9 is not only involved in innate immunity but also in adaptive immunity and can be used as alternative adjuvant development research.
Keywords: antigenic protein, CD4, CD8, immunoglobulin G, Sarcoptes scabiei var. caprae, toll-like receptor-9 |
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Article PeerReviewed |
author |
Nunuk Dyah Retno Lastuti, - Wiwik Misaco Yuniarti, - Poedji Hastutiek, - Lucia Tri Suwanti, - Dony Chrismanto, - |
author_facet |
Nunuk Dyah Retno Lastuti, - Wiwik Misaco Yuniarti, - Poedji Hastutiek, - Lucia Tri Suwanti, - Dony Chrismanto, - |
author_sort |
Nunuk Dyah Retno Lastuti, - |
title |
Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
title_short |
Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
title_full |
Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
title_fullStr |
Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
title_full_unstemmed |
Humoral and cellular immune response induced by antigenic protein of Sarcoptes scabiei var. caprae |
title_sort |
humoral and cellular immune response induced by antigenic protein of sarcoptes scabiei var. caprae |
publisher |
Veterinary World |
publishDate |
2018 |
url |
https://repository.unair.ac.id/102546/3/Bukti%20C%2003%20Humoral%20and%20celluler%20immune.pdf https://repository.unair.ac.id/102546/6/C03_Peer_Review_Lucia_Tri_Suwanti.pdf https://repository.unair.ac.id/102546/1/Turnitin%20C%2003.%20Humoral%20and%20cellular%20immune%20response%20....pdf https://repository.unair.ac.id/102546/ |
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1733034077816094720 |