Treated Plasmodium berghei infected pregnant mice by Andrographis paniculata tablet (AS201-01) decreasing the TLR-4 expression and apoptosis index of placental tissue

• Main Authors: Budi Prasetyo, -, Diantri Nari Ratih, -, Yustinasari, -, Hilkatul Ilmi, -, Lidya Tumewu, -, Aty Widyawaruyanti, -
• Format: Article PeerReviewed
• Language: English; English; English; English
• Published: MediPoeia 2018
• Subjects: R Medicine; RS Pharmacy and materia medica; RS1-441 Pharmacy and materia medica; RS200-201 Pharmaceutical dosage forms
• Online Access: https://repository.unair.ac.id/102700/1/C17-ARTIKEL.pdf; https://repository.unair.ac.id/102700/2/C17-PEER%20REVIEW.pdf; https://repository.unair.ac.id/102700/3/14%25%20Treated%20Plasmodium%20berghei%20infected%20pregnant%20mice%20by%20Andrographis%20paniculata%20tablet%20%28AS201-01%29%20decreasing%20the%20TLR-4%20expression%20and%20apoptosis%20index%20of%20placental%20tissue.pdf; https://repository.unair.ac.id/102700/5/C-17-KORESPONDENSI.pdf; https://repository.unair.ac.id/102700/; https://www.japsonline.com/abstract.php?article_id=2607&sts=2
• Institution: Universitas Airlangga

Objective: This study aimed to evaluate the effect of Andrographis paniculata tablet (AS201-01) in decreasing expression of Toll-Like Receptor-4 (TLR-4) and apoptosis index of placental tissue of Plasmodium berghei infected pregnant mice. Material and Methods: A total of 24 pregnant mice were divided into 4 groups, G1 was uninfected pregnant mice group, G2 was untreated Plasmodium berghei infected pregnant mice, G3 was P. berghei infected pregnant mice treated with AS201-01 tablet and G4 was P. berghei infected pregnant mice treated with dihydropiperaquine-phosphate (DHP) tablets. All mice were sacrifice at day 15th of pregnancy and the placenta was collected. The TLR-4 expression and apoptosis index were evaluated using immunohistochemistry assay. Results: The TLR-4 expression and apoptosis index on G3 was lower than G2 and showed a statistically significant difference (P = 0.00). The TLR-4 expression and apoptosis index of placental tissue in P. berghei infected pregnant mice which treated with AS201-01 tablet were decreased compared to untreated group and DHP tablet treated group as well. Conclusion: AS201-01 tablet containing ethyl acetate fraction of A. paniculata could decrease TLR-4 expression and apoptosis index of placental tissue in P. berghei infected pregnant mice.